| 海绵polymistia spongia提取物aldisin的抗脂质过氧化及清除自由基作用研究 |
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| 引用本文： | 唐孝礼,许实波,徐石海.海绵polymistia spongia提取物aldisin的抗脂质过氧化及清除自由基作用研究[J].中国药理学通报,1998(2). |
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| 作者姓名： | 唐孝礼 许实波 徐石海 |
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| 作者单位： | 中山大学药学系(唐孝礼，徐石海)，暨南大学化学系(许实波) |
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| 摘 要： | 目的研究海绵ｐｏｌｙｍｉｓｔｉａｓｐｏｎｇｉａ提取物ａｌｄｉｓｉｎ的抗脂质过氧化及清除自由基作用。方法采用ＭＤＡ－ＴＢＡ比色法测定组织匀浆中过氧化脂质（ＬＰＯ）含量；采用邻苯三酚自氧化法测定ａｌｄｉｓｉｎ对Ｏ·２自由基的清除作用；采用分光光度法测定ａｌｄｉｓｉｎ对Ｃｕ２＋－ＶｉｔＣ体系产生的·ＯＨ自由基的清除作用；采用ＥＳＲ法测定ａｌｄｉｓｉｎ对ＤＰＰＨ自由基的清除作用。结果ａｌｄｉｓｉｎ具有显著的抗脂质过氧化作用，并能有效地清除Ｏ·２、·ＯＨ和ＤＰＰＨ自由基，在０．４２６μｍｏｌ·Ｌ－１～１８．５ｍｍｏｌ·Ｌ－１浓度范围内有明显的量效关系。结论ａｌｄｉｓｉｎ是自由基清除剂。
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| 关 键 词： | 海绵 aldisin 脂质过氧化 自由基 |
Studies on the effects of anti lipoperoxidation and radical scavenging action of aldisin from polymistia spongia |
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| Abstract: | AIM To study the effects of anti lipoperoxidation and free radical scavenging action of aldisin from polymistia spongia. METHODS LPO content in tissue homogenerate was measured by MDA TBA spectrophotometry. The scavenging action on oxygen free radical (O·2) was determined by pyrogallol auto oxidation method. The scavenging action on hydroxygen free radical (·OH) produced by Cu2+ VitC system was determined by spectrophotometry. And that on DPPH radical by ESR method. RESULTS aldisin had remarkable anti lipoperoxidation and could effectively scavenge O·2, ·OH and DPPH radicals. The effects of aldisin were obviously dose dependent within the extent of 0426 μmol·L-1￣185 mmol·L-1.CONCLUSION aldisin was a kind of free radical scavenger |
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| Keywords: | polymistia spongia aldisin lipid peroxidation free radicals |
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