| HIV/AIDS患者机体氧化还原态与CD4细胞计数相关性分析 |
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| 引用本文： | 韩晓群,吴建红,伍仕敏,熊瑛,熊悦安.HIV/AIDS患者机体氧化还原态与CD4细胞计数相关性分析[J].武汉大学学报(医学版),2012,33(3):401-403,448. |
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| 作者姓名： | 韩晓群 吴建红 伍仕敏 熊瑛 熊悦安 |
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| 作者单位： | 韩晓群 (武汉市医疗救治中心,湖北武汉,430023) ； 吴建红 (武汉市医疗救治中心,湖北武汉,430023) ； 伍仕敏 (武汉市医疗救治中心,湖北武汉,430023) ； 熊瑛 (武汉市医疗救治中心,湖北武汉,430023) ； 熊悦安 (武汉市医疗救治中心,湖北武汉,430023) ； |
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| 基金项目： | 武汉市卫生局科研基金资助项目 |
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| 摘 要： | 目的:探讨HIV/AIDS患者机体氧化还原态与CD4细胞计数的相关性。方法:采用流式细胞仪检测CD4+和CD8+计数,计算CD4+/CD8+比值。将患者分为AIDS组(54例)和HIV感染组(26例),正常体检者为对照组(30例)。取静脉血,测血还原型谷胱甘肽(GSH)与氧化型谷胱甘肽(GSSG),NADPH与NADP+,以GSH/GSSG、NADPH/NADP+比值表示患者机体氧化还原态,分析氧化还原态与CD4细胞计数的相关性。结果:HIV/AIDS患者血CD4细胞计数及CD4+/CD8+比值均随机体氧化还原态向氧化方向偏移而减少。结论:AIDS/HIV患者机体氧化还原态越向氧化方向偏移,标志着AIDS患者病情进展。
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| 关 键 词： | HIV/AIDS 氧化还原态 CD4+ 相关性分析 |
Correlation Between Redox Status and Disease Severity in HIV/AIDS Patients |
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| Institution: | HAN Xiaoqun,WU Jianhong,WU Shimin,XIONG Ying,XIONG Yue’an Wuhan Medical Treatment Center,Wuhan 430023,China |
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| Abstract: | Objective: To study the correlation between redox status and CD4+ count in HIV/AIDS patients.Methods: CD4+ and CD8+ T lymphocyte counts were detected by flow cytometry.AIDS cases(n=54),HIV infected cases(n=26),and normal subjects(n=30) were involved in this study.Plasma glutathione(reduced form GSH and oxidized form GSSG) were measured by fluorescence spectrophotometry,NADPH(reduced form) and NADP+(oxidized form) were measured by UV spectrophotometry.Redox status was expressed by GSH/GSSG and NADPH/NADP+ and the correlation between redox status and CD4+ count was analyzed.Results: CD4+ count and CD4+/ CD8+ ratio in blood of HIV/AIDS patients decreased with redox status in plasma deviated to oxidative direction.Conclusion: Redox status in plasma of HIV/AIDS deviates more to oxidative direction,diseases become more to progression. |
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| Keywords: | HIV/AIDS Redox Status CD4+ Analysis of Correlation |
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