淋球菌抗生素耐药谱和质粒图谱研究

目的 为了解广州地区淋球菌的性素耐药谱和质粒谱。方法 采用琼脂稀释法测定了５种抗生素对８９株淋球菌的最小抑菌浓度（ＭＩＣ）和碱裂解法分析１０６株菌的质粒衅谱。结果 ２株为产β－内酰胺酶菌株（ＰＰＮＧ），３株为质粒介导的高度耐四环素菌株９ＴＲＮＧ０；环丙沙星耐药率较高（６２．９％），青霉素次之（５９．５％），头孢三嗪和壮观霉素未发现耐药菌株。质粒检出率为９０．６％，其中２．６Ｍｄ隐蔽质粒携带率为８５．６％，４．５ＭｄＲ质粒为６７．８％，２４．５Ｍｄ结合质粒为３３．８％。质粒谱型以２．６Ｍｄ＋４．５Ｍｄ和２．６Ｍｄ＋４．５＋ｉ２４．５Ｍｄ多见，分别为３７．７％和２５．４％。结论 表明了广州地区淋球菌抗生素耐药谱和质粒图谱，有助于淋病的治疗和防治。

Study on Antibiotic Susceptibility Patterns and Plasmid Profiles of Neisseria gonorrhoeae

Objective To survey the antibiotic susceptibilities and plasmid profiles of Neisseria gonorrhoeae in Guangzhou. Methods Minimum inhibitory concentrations(MIC) to gonorrhoeae of penicillin G, tetracycline, ciprofloxacin, ceftriaxone and specti- nomycin was determined using the agar dilution method. Plasmid DNA was obtained by the alkaline lysis method and profiles generat- ed by UVI digital imaging and analysis systems. Results 2 strains of Neisseria gonorrhoeae produced lactamase, while 3 showed plasmid- mediated, high - level resistance to tetracycline(TRNG). Resistant rates of ciprofloxacin and penicillin were 62. 9 % and 59. 5 %, respectively, but there was no resistance to ceftriaxone and specitinomycin. Out of 106 strains, 96 (90. 6 % ) contained plasmids: 85. 6% possessed 2. 6Md cryptic plasmid, 67. 8% 4. 5Md R palsmid and 33. 8% 24. 5Md conjugative plasmid. The more prevalent profiles constained 2. 6 4. 5Md(37. 7% ) and 2. 6 4. 5 24. 5Md(25. 4 % ) plasmids. Conclusion The study showed antibiltic susceptibility patterns and plasmid profiles of Neisseria gonorrhoeae in Guangzhou, which would be helpful in treatment and control of Gonorrhea.