基于网络药理学探讨红萸饮治疗溃疡性结肠炎的分子机制

目的:采用网络药理学的方法,对红萸饮(HYY)治疗溃疡性结肠炎(UC)的作用机制进行探讨.方法:通过TCMSP平台和Uniprot数据库对HYY进行活性成分及作用靶点筛选和标准化;在Genecards和在线人类孟德尔遗传数据库中获取疾病靶点.采用Draw Venny Diagram程序生成药物活性成分-疾病靶点数据集及...

Mechanism of Hongyu Drink in the Treatment ofUlcerative Colitis Based on Network Pharmacology

Objective:To explore the mechanism of the action of Hongyu Drink(HYD)in the treatment on ulcerative colitis(UC)by network pharmacology.Methods:The active ingredients and targets of HHD were screened by TCMSP platform,and the targets′name of the selected targets were standardized by Uniprot database.The search was performed in Genecards database and online human Mendelian genetic database to obtain disease target points.The Draw Venny Diagram online program was used to generate the pharmaceutically active ingredient-disease target data set and the Venn diagram.Protein-protein interaction network map was generated by using String website;component-target network was constructed by using Cytoscape 3.7.2 software;GO function annotation and KEGG pathway enrichment analysis were performed by R3.6.0 software.The C57 UC mouse model was established by DSS,and HE staining、ELIS、qPCR were used to verify the results of network pharmacology detection.Results:A total of 50 kinds of drug components were included in the 4 herbs of HHY;110 potential targets were predicted;the total number of UC-related disease targets was 3870;a total of 70 common targets of Pulsatilla chinensis-disease were obtained,and the co-targets were mainly enriched in 88 biological processes and 113 signaling pathways.The experimental results showed that HYDin can improve the colorectal epithelial integrity of UC animal model and reduce the expression of VEGF mRNA in serum IL-6 and colorectal tissues.Conclusion:Combined with experiment,network pharmacology analysis shows that HYDin may regulate the CASP3,IL-6,VEGFA,et al,and PI3K-Akt signaling pathway,et al,which regulates apoptosis,angiogenesis and inhibition of inflammation,achieves a therapeutic effect on ulcerative colitis.Other pathways need our further study.