清肠化湿方对溃疡性结肠炎小鼠炎症及肠道菌群的影响

目的 探究清肠化湿方对溃疡性结肠炎模型小鼠的治疗作用及机制.方法 DSS诱导制备溃疡性结肠炎小鼠模型,称小鼠体质量,量取结肠长度,制备结肠病理切片,ELISA法检测结肠组织中IL-6和TNF-α的水平,气相色谱-质谱法分析测定结肠肠道内容物中丁酸、异丁酸的含量,基于16 S rRNA扩增与高通量测序技术检测分析小鼠粪便...

Effects of Qingchang Huashi Decoction on Inflammation and Gut Flora in Ulcerative Colitis Mice

OBJECTIVE To explore the treatment and mechanism of Qingchang Huashi Decoction on ulcerative colitis mice.METHODS The mouse model of ulcerative colitis was induced by DSS,and the mice were treated with drug intervention treatment and weighed during the experiment.At the end of the experiment,the length of the colon was measured,and pathological sections of the colon were made.The contents of IL-6 and TNF-α in colon tissue were detected by enzyme-linked immunoassay.Gas chromatography-mass spectrometry was used to determine the concentrations of butyric acid and isobutyric acid in the contents of the colon.High throughput sequencing technology based on 16S rRNA amplification was used to detect and analyze the composition and abundance of mouse fecal flora.RESULTS Compared with the healthy control group,mice with ulcerative colitis showed weight loss,enlarged spleen,shortened colon length(P<0.05,P<0.01),increased levels of IL-6 and TNF-α in colon tissue(P<0.01),decreased butyric acid and isobutyric acid in colon contents(P<0.01),and changed gut flora composition and abundance.After treatment with Qingchang Huashi Decoction,the weight of the mice was increased significantly,the spleen enlargement was reduced,and the length of the colon was increased.Compared with the model group,the colon pathological damage was ameliorated and the levels of IL-6 and TNF-α in colon tissue were reduced.After treatment,the contents of butyric acid and isobutyric acid in the colon contents increased significantly,and the unbalanced gut flora was adjusted to approaching the normal state.CONCLUSION The mechanism of Qingchang Huashi Decoction in treating ulcerative colitis is related to its regulating the gut flora,increasing the contents of butyric acid and isobutyric acid in the colon,and reducing the levels of pro-inflammatory factors including IL-6 and TNF-α.