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| 人参皂苷Rg_1对大鼠海马c-fos基因表达和cAMP含量的影响(英文) | |
| 引用本文： | 刘忞,张均田. 人参皂苷Rg_1对大鼠海马c-fos基因表达和cAMP含量的影响(英文)[J]. Acta pharmacologica Sinica, 1996, 0(2) |
| 作者姓名： | 刘忞 张均田 |
| 作者单位： | 中国医学科学院药物研究所，中国医学科学院药物研究所 北京 100050 中国，北京 100050 中国 |
| 基金项目： | supported by the National Natural Science Foundation of China,№ 39370796. |
| 摘 要： | 目的:探讨Rg_1对神经系统作用的机制。方法:采用Northern和Western印迹分析法,检测了Rg_1处理前后大鼠海马组织的c-fos基因和蛋白的表达。结果:老年鼠c-fos基因和蛋白的表达明显低于青年鼠,但给Rg_1后老年鼠和青年鼠均呈现显著性增强效应。此外,Rg_1还明显增加青年鼠和老年鼠海马组织的cAMP含量。结论:Rg_1升高cAMP水平及促进c-fos基因和蛋白的表达有助于阐明其促智和抗衰老作用的机制。 |
| 关 键 词： | 人参 皂苷类 fos基因 原癌基因蛋白c-fos 腺苷环一磷酸 RNA印迹 蛋白质印迹 海马 |
Effects of ginsenoside Rg1 on c-fos gene expression and cAMP levels in rat hippocampus |
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| LIU Min,ZHANG Jun-Tian. Effects of ginsenoside Rg1 on c-fos gene expression and cAMP levels in rat hippocampus[J]. Acta pharmacologica Sinica, 1996, 0(2) | |
| Authors: | LIU Min ZHANG Jun-Tian |
| Abstract: | AIM: To study the mechanisms of Rg1 antiaging and nootropic function. METHODS: Using Northern and Western blot analyses, the levels of c-fos mRNA and fos protein were determined in the hippocampus of young and old rats treated with or without ginsenoside Rg1. RESULTS : The expression of c-fos gene and protein was decreased in the hippocampus of aged rats, but dose-dependently increased in young and aged rats after the administration of Rg1. Furthermore, Rg1 increased the level of cAMP in the hippocampus of both young and old rats. CONCLUSION: The changes at the genomic and protein levels, arisen from the increase of cAMP, provide an explanation of the mechanisms of Rg1 nootropic and antiaging function. |
| Keywords: | ginseng saponins fos genes proto-oncogene proteins c-fos cyclic AMP. North- ern blotting Western blotting hippocampus |
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