流式细胞术分析肺癌患者外周血细胞的免疫变化

目的 :研究肺癌及肺部一般炎症患者外周血细胞免疫的变化及临床意义。方法 :采用流式细胞术 (FCM )对35例原发性肺癌、38例肺部一般炎症患者的外周血CD3、CD4 、CD8、CD16+ 56进行定量检测。结果 :恶性肿瘤患者外周血CD4 、CD4 /CD8、CD3、CD15+ 16均显著性低于健康对照组 (P <0 0 1) ;而CD8则上升 (P <0 0 5 ) ,均有统计学意义。肺部一般炎症患者外周血CD3、CD4 、CD4 /CD8较健康人降低 ,CD8则升高 ,但无显著性差异 (P >0 0 5 ) ,而CD16+ 56较健康人也降低 ,但 ( 0 0 1

Using FCM to analyse the change of the immanocyte in peripheral blood of the patients with lung cancer

Objective To investigate immune function of the cell in the peripheral blood and it's meaning in the clincial practice,who suffer from lung cancer or common inflammation of lung.Methods:Using flow cytometry and monclonal antibodies to evaluate T lymphocyte subsets NK cells of peripheral blood in 30 normal,35 patients with lung cancer and 38 patients with common inflammation of lung.Results:The CD 3,CD 4 T cells and helper/suppressor radio (CD 4/CD 8),CD 56+16 were significantly lower (P<0 01) and CD 8 T cells was significantly higher (P<0 05) in patients with lung cancer than in normal controls.The CD 3,CD 4,CD 8 T cells and helper/suppressor radio (CD 4/CD 8) were no significant difference in patients with common inflammation of lung to the normal controls (P>0 05),but CD 56+16 low have difference (0 01