近部与远部穴位埋线对原发性痛经大鼠子宫前列腺素、血清IL-2及脾脏NK细胞的影响

目的:观察近部与远部穴位埋线对原发性痛经(PD)大鼠子宫组织前列腺素E2(PGE2)和前列腺素F2α(PG F2α)含量、血清白细胞介素-2(IL-2)含量、脾脏自然杀伤(N K)细胞活性的影响,探讨近部与远部穴位埋线对PD大鼠的效应差异.方法:雌性Wistar大鼠随机分为对照组、模型组、近部埋线组、远部埋线组,每组8...

Effect of proximal and distal acupoint catgut-embedding on uterus prostaglandin,serum IL-2 and splenic NK cell activity in primary dysmenorrhea rats

Objective To observe the effect of proximal and distal acupoint catgut-embedding on the contents of prostaglandin E2(PGE2) and prostaglandin F2α(PGF2α) in the uterus, serum Interleukin-2(IL-2) contents and splenic natural killer(NK) cell activity in primary dysmenorrhea(PD) rats, so as to explore the different effects of proximal and distal acupoint catgut-embedding on PD rats. Methods Female Wistar rats were randomly divided into control, model, proximal catgut-embedding and distal catgut-embedding groups, with 8 rats in each group. The PD model was established by subcutaneous injection of estradiol ben-zoate(0.5 mg/rat on the first day and 10th day, and 0.2 mg/rat from the 2nd to the 9th day) and intraperitoneal injection of oxytocin(2 U/rat, on the 11th day). Catgut-embedding was applied at bilateral "Guanyuan"(CV4) and "Ciliao"(BL32) in the proximal catgut-embedding group, and bilateral "Sanyinjiao"(SP6) and "Diji"(SP8) in the distal catgut-embedding group. After the treatment, the body writhing times in 30 min and uterine mass were recorded, PGE2 and PGF2αin uterus and IL-2 in serum were assayed by using ELISA, the activity of NK cell in the spleen was detected using MTT colorimetry. Results Following modeling, the body writhing times of the model group was increased than that of the control group(P<0.01);After interventions, the body writhing times were decreased in the proximal and distal catgut-embedding groups than those of the model group(P<0.01). Compared with the control group, the uterine mass and uterus PGF2αcontent were increased(P<0.01), while uterus PGE2, serum IL-2 and splenic NK cell activity were decreased(P<0.01) in the model group. After interventions, the uterine mass and uterus PGF2α were down-regulated(P<0.01, P<0.05), while the contents of uterus PGE2, serum IL-2 and splenic NK cell activity up-regulated(P<0.01) in the proximal and distal catgut-embedding groups in contrast to the model group. The content of uterus PGF2α was down-regulated(P<0.05) and splenic NK cell activity was up-regulated(P<0.01) in the proximal catgut-embedding group than those of the distal catgut-embedding group. Conclusion Both proximal and distal acupoint catgut-embeding can increase PGE2 and decrease PGF2α in the uterus, increase the level of serum IL-2 and the activity of NK cells in the spleen in PD model rats, thereby achieving analgesic effect. The effect of proximal catgut-embedding is better than that of distal catgut-embedding.