瓜蒌提取物抗氧化药效成分群的筛选与验证

目的 基于均值化邓氏关联度计算法研究瓜蒌提取物抗氧化的物质基础并进行抗氧化药效成分群的筛选与验证。方法 采用HPLC法建立瓜蒌提取物的指纹图谱，测定瓜蒌提取物（27、9、3 mg/mL）对DPPH自由基（DPPH·）、超氧阴离子自由基（O2-·）清除率，采用均值化邓氏关联度计算法研究瓜蒌提取物抗氧化的谱效关系并筛选抗氧化药效成分群。按瓜蒌提取物抗氧化药效成分群中已知成分的含量制备混合对照品溶液并测定DPPH·、O2-·清除率。结果 瓜蒌提取物HPLC指纹图谱中36个共有峰与DPPH·、O2-·清除率间的关联度不同，关联度大于中位数值的共有峰为：21、36、8、31、14、5、27、2、24、15、18、33、22、34、35、19、28、25号峰，初步认定山奈酚（36号峰）、异槲皮苷（8号峰）、木犀草素（31号峰）、芦丁（5号峰）、芹菜素（35号峰）5个成分为瓜蒌提取物抗氧化药效成分群（简称抗氧化药效成分群），山奈酚：异槲皮苷：木犀草素：芦丁：芹菜素=3∶2∶2∶1∶1（质量比）。抗氧化药效成分群（6.12、2.04、0.68 μg/mL）对DPPH·清除率分别为65.4%、64.0%、61.0%，对O2-·清除率分别为12.9%、9.5%、8.3%。结论 明确了瓜蒌提取物抗氧化的物质基础并筛选出了其抗氧化药效成分群。

Screening of effective antioxidant components from Trichosanthes extract and assessment of their antioxidant activity

Objective To screen the effective antioxidant components in Trichosanthes extract based on the mean value of Deng's correlation degree and assess the antioxidant activity of the identified components. Method High-performance liquid chromatography (HPLC) was used to obtain the fingerprints of Trichosanthes extract, and the clearance rates of DPPH · and O2-· by 3, 9 and 27 mg/mL Trichosanthes extract were determined. The antioxidant spectrum effect of Trichosanthes extract was analyzed by calculating the mean value of Deng's correlation degree to screen the effective antioxidant component group. According to the contents of each known components in the antioxidant effective component group, mixed solutions of the components were prepared and tested for their clearance rates of DPPH · and O2-· . Results The 36 common peaks in HPLC fingerprints of Trichosanthes extract showed different degrees of correlation with DPPH · and O2-· clearance. The common peaks with a correlation degree greater than the median value included peaks 21, 36, 8, 31, 14, 5, 27, 2, 24, 15, 18, 33, 22, 34, 35, 19, 28 and 25. The 5 components, namely kaempferol (peak 36), isoquercitrin (peak 8), luteolin (peak 31), rutin (peak 5) and apigenin (peak 35), were tentatively identified to constitute the effective antioxidant component group with a mass ratio 3∶2∶2∶1∶1 in Trichosanthes extract. The prepared mixed solutions of antioxidant effective component group (6.12, 2.04, and 0.68 μg/mL) showed clearance rates of DPPH· of 65.4%, 64.0% and 61.0%, and clearance rates of O2-· of 12.9%, 9.5% and 8.3%, respectively. Conclusion We identified the material basis for the antioxidant activity of Trichosanthes and screened the antioxidant effective component group in Trichosanthes extract.