苦地丁总生物碱对小鼠Ⅱ型胶原关节炎的影响

目的探讨苦地丁对小鼠Ⅱ型胶原关节炎的影响。方法小鼠尾根部皮内注射Ⅱ型胶原乳剂建立小鼠关节炎模型(CIA),苦地丁总生物碱(50,100,200mg·kg-1组灌胃给药,每日1次;模型组和空白组灌胃给予等量蒸馏水;实验结束时考察各组临床积分、发病时间、血清中抗-CⅡ抗体含量、胶原所致耳廓DTH;ELISA法检测PGE2、TNF-α、IL-1的水平。结果苦地丁剂量依赖性延迟CIA小鼠的发病时间,减少关节的肿胀和红斑,缓解CIA小鼠体质量减轻,降低小鼠血清中抗-CⅡ抗体水平,抑制CⅡ诱导的小鼠耳廓迟发型超敏反应;明显抑制巨噬细胞释放肿瘤坏死因子(TNF-α)、白介素1(IL-1),但对前列腺素(PGE2)的产生无明显影响。结论苦地丁对小鼠CIA有明显的抑制作用。

Effect of total alkaloids from Cory dalis bengeana Turcz .on collagen-induced ar-thritis in mice

Objective To investigate the therapeutic effects of Corydalis bengeana Turcz .on collagen Ⅱ-induced arthritis（CIA） in mice .Methods The CIA model was established by intradermal injection of type Ⅱ collagen emulsion into mice tail .The total al-kaloids from C .bengeana （50 ,100 ,200 mg·kg -1 ） were orally administrated daily .Model group and control group mice were ad-ministrated with saline .At the end of the experiment groups ,clinical integral ,onset time ,anti-C Ⅱ antibodies in the serum , DTH ,macrophage proliferation and activities of tumor necrosis factor-alpha（TNF-α） ,interleukin-1（IL-1） ,prostaglandin E2 （PGE2 ） were respectively determined .Results The results showed that the total alkaloids dose-dependently delayed the onset time of CIA ,diminished the swelling and erythema on the joints ,relieved the loss of body weight ,decreased the titer of anti-CⅡantibody in serum ,suppressed the delayed type hypersensitivity（DTH） against CⅡ in ears .The total alkaloids also inhibited the release of TNF-α,IL-1 from macrophages ,but did not affect the production of PGE2 .Conclusion C .bengeana has a significant inhibiting effect on CIA .