Investigation on the morphological protective effect of 5-hydroxymethylfurfural extracted from wine-processed Fructus corni on human L02 hepatocytes |
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| Authors: | Ming-Yan Wang Feng-Ming Zhao Hai-Yan Peng Cheng-Hua Lou Yu Li Xia Ding Xiao-Yi Yu Guang-Ming Yang Dong-Qing Xu Li-Hua Jiang Xu Zhang Li-Hong Ye Bao-Chang Cai |
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| Affiliation: | 1. Jiangsu Key Laboratory of Chinese Medicine Processing, Level-3 Laboratory of State Administration of TCM of the People''s Republic of China, Nanjing University of Chinese Medicine, Nanjing 210029, China;2. Nanjing Agricultural University, Nanjing 210095, China;3. China Pharmaceutical University, Nanjing 210009, China |
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| Abstract: | AimTo determine the mode of action of 5-hydroxymethylfurfural (5-HMF) extracted from wine-processed Fructus corni on hepatoprotective activities, the effects of 5-HMF on H2O2-induced human L02 hepatocytes injury was examined.MthodsHepatocytes L02 injured by H2O2 was treated by 5-HMF. The morphological changes of the cells were observed under inverted phase-contrast, fluorescence, and transmission electron microscopy and the activities of caspase-9 and caspase-3 were tested by enzyme-linked immunosorbent detector.ResultsIt revealed that 5-HMF improved the morphology of H2O2-treated human L02 hepatocytes, and also inhibited the level of caspase-9 and caspase-3 of them.ConclusionsThese results suggested a morphological hepatocyte protective effect and the anti-apoptosis mechanism by 5-HMF. |
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| Keywords: | 5-HMF H2O2 L02 cells Fructus corni |
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