| 化学发光免疫定量检测狂犬病毒抗体方法的建立 |
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| 引用本文： | 宋琳 郭兰英. 化学发光免疫定量检测狂犬病毒抗体方法的建立[J]. 临床检验杂志, 2006, 24(2): 89-90 |
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| 作者姓名： | 宋琳 郭兰英 |
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| 作者单位： | 华美生物工程公司,河南,洛阳,471003;华美生物工程公司,河南,洛阳,471003 |
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| 摘 要： | 目的建立化学发光免疫法检测人血清中狂犬病毒抗体。方法以纯化抗原包被固相,配以碱性磷酸酶标记抗原及金刚烷胺作为发光底物,采用双抗原夹心法检测血清中抗体。结果该方法的敏感性为0.2 IU/m l,线性范围为0~200 IU/m l,变异系数<10%,回收率在90%~110%之间。结论化学发光法定量测定人血清中狂犬病毒抗体是一种灵敏、准确、重复性好,操作简单的方法,适用于临床检测。
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| 关 键 词： | 狂犬病毒 化学发光 酶联免疫吸附性试验 |
| 文章编号： | 1001-764X(2006)02-0089-02 |
| 收稿时间： | 2005-05-12 |
| 修稿时间： | 2005-09-28 |
Quantitative detection of Rabies virus antibody by chemiluminescent immunoassay |
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| SONG Lin,GUO Lanying. Quantitative detection of Rabies virus antibody by chemiluminescent immunoassay[J]. Chinese Journal of Clinical Laboratory Science, 2006, 24(2): 89-90 |
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| Authors: | SONG Lin GUO Lanying |
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| Affiliation: | Sino-American Biotechnology Company, Luoyang 4701003, China |
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| Abstract: | Objective To establish chemiluminescent immunoassay(CLIA) for quantitative detection of Rabies virus antibody in human serum.Method The purified antigen was coated on the microplate,pairing with alkaline phosphatase(ALP)-labeled antigen and luminescence substrate CSPD,then the double antigen sandwich CLIA was established.Results The sensitivity of the assay was 0.2IU/ml.The linear range was from 0 IU/ml to 200 IU/ml.The precision was less than 10%.The analytical recovery rate was from 90% to 110%.Conclusion The CLIA is a simple,sensitive,special and repeatable method for detection of Rabies virus antibody.It was suitable for clinical application. |
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| Keywords: | Rabies virus chemiluminescent immunoassay enzyme linked immunoadsorbent assay |
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