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| 金黄色葡萄球菌分泌趋化抑制蛋白与C5aR、C5L2的相互作用 | |
| 引用本文： | 刘振嘉,杜冠华,宫丽丽,高金明. 金黄色葡萄球菌分泌趋化抑制蛋白与C5aR、C5L2的相互作用[J]. 基础医学与临床, 2010, 30(5): 496-499 |
| 作者姓名： | 刘振嘉 杜冠华 宫丽丽 高金明 |
| 作者单位： | 1. 中国医学科学院,北京协和医学院北京协和医院呼吸内科,北京,100730 2. 中国医学科学院,北京协和医学院药物研究所,北京,100050 |
| 基金项目： | 北京市自然科学基金(7072063) |
| 摘 要： | 目的探讨金黄色葡萄球菌分泌的趋化抑制蛋白(CHIPs)与G蛋白偶联受体(GPCR)C5a受体(C5aR)或促酰化蛋白受体(C5L2))的相互作用。方法将纯化的CHIPs蛋白分别与表达C5aR、C5L2、CXC趋化因子受体3(CXCR3)的人肾胚胎上皮细胞293T分别孵育,用Western blot检测结合情况。结果CHIPs蛋白与C5aR相结合,与C5L2和CXCR3无明显结合。结论C5aR是CHIPs蛋白的受体,提示C5L2与C5aR在和CHIPs的结合作用上存在明显差异。 |
| 关 键 词： | CHIPs C5aR C5L2 相互作用 |
| 收稿时间： | 2009-11-05 |
| 修稿时间： | 2010-02-01 |
The interaction between C5aR,C5L2 and chemotaxis inhibitory protein secreted by Staphylococcus aureus |
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| LIU Zhen-jia,DU Guan-hua,GONG Li-li,GAO Jin-ming. The interaction between C5aR,C5L2 and chemotaxis inhibitory protein secreted by Staphylococcus aureus[J]. Basic Medical Sciences and Clinics, 2010, 30(5): 496-499 | |
| Authors: | LIU Zhen-jia DU Guan-hua GONG Li-li GAO Jin-ming |
| Affiliation: | 1.Dept.of Respiration;PUMC Hospital;CAMS & PUMC;Beijing 100730;2.Institute of Materia Medical;Beijing 100050;China |
| Abstract: | Objective To investigate the mutual effect between G protein-coupled receptors(GPCR) including C5a receptor(C5aR),C5a like receptor 2(C5L2) and chemotaxis inhibitory protein(CHIPs) secreted by Staphylococcus aureus.Methods The purified CHIPs was incubated with HEK 293T cells overexpressing C5aR,C5L2 and C-X-C chemokine receptor type 3(CXCR3).Binding signal was detected by Western blot.Results HEK 293T cells overexpressing C5aR can efficiently bind to CHIPs.No apparent relation between CHIPs and C5L2 or CXCR... |
| Keywords: | CHIPs C5aR C5L2 interaction |
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