新疆居民HBV基因型的分布及其与临床表现关系的初步研究

目的 了解新疆地区乙肝患者HBV基因型的分布及其与临床表现的关系.方法 从2000余份血清中筛选出HBsAg阳性血清200份.利用巢式PCR法扩增血清中HBV DNA S基因进行测序并分型;统计分析基因型分布及与临床表现的关系.结果 新疆HBV有B、C、D三个基因型,其中B基因型10例（7.8%）,C基因型58例（45.7%）,D基因型59例（46.5%）.C基因型中汉族51例（87.9%）,D型中汉族27例（45.7%）,维吾尔族24例（40.7%）.结论 新疆地区HBV基因型以C、D型为主,少量为B型.C型以汉族为主,D型中汉族和维吾尔族占多数.C型与更严重的疾病及转归有关.

Preliminary study on distribution and relationship with phenotype of hepatitis B virus genotype detected from Xinjiang uygur autonomous region in China

Objective To determine the main genotype of hepatitis B virus (HBV) in Xinjiang.Methods 200 HBsAg positive serum specimens were detected from more than 2000 serum of Xinjiang inhabitants, and HBV S gene was detected by using nPCR amplifying, and compared with the standard S region HBV nucleotide sequences of genotypes A-H retrieved from GenBank, then analyzed by MEGA3 and SAS3 software. Result Gene in 127 (63.5%) serum specimens was detected from 200 samples. Among 127 serum specimens, 10 (7.8%) was genotype B, 58 (45.7%) was genotype C, and 59 (46.5%) was genotype D. Han People were 51 (87.9%) in genotype D and 27(45.7%) in genotype D, Uygur were 24 in genotype D. Conclusion Genotype B(40.7%), C and D have been found in Xinjiang. Genotype C was more prone to causing severe liver disease.