50株耐亚胺培南的鲍曼不动杆菌和铜绿假单胞菌NDM-1基因的筛查

目的检测耐亚胺培南鲍曼不动杆菌和铜绿假单胞菌NDM-1基因,了解本院NDM-1超级细菌的流行情况。方法从2011年3-9月期间分离的1 430株细菌中挑出耐亚胺培南50株非发酵菌(34株鲍曼不动杆菌,16株铜绿假单胞菌),采用卫生部推荐产NDM-1酶细菌的实验室诊断方法进行操作,包括K-B法、改良Hodge试验、PCR扩增法。结果通过K-B法,50株菌株亚胺培南抑菌圈直径≤10 mm;经改良Hodge试验后得出50株待测菌为碳青霉烯酶阴性;PCR法得到的扩增产物进行琼脂糖凝胶电泳,未显示目的基因条带。结论产NDM-1酶的超级细菌在本院暂不流行。

Detection of NDM-1 Gene in 50 Strains of Imipenem-resistant Acinetobactor baumannii and Pseudomonas aeruginosa

Objective To screen NDM-1 gene in imipenem-resistant Acinetobactor baumannii and Pseudomonas aeruginosa and to understand the epidemiology of NDM-1 superbugs in the second Xiangya Hospital of Central South University.Methods Fifty strains of non-fermentative bacteria,including 34 strains of Acinetobacter baumannii and 16 strains of Pseudomonas aeruginosa,were selected from 1,430 strains of bacteria isolated from the clinics in the Second Xiangya Hospital of Central South University during March to September in 2011.The NDM-1 enzyme-producing superbugs were detected according to the laboratory diagnosis methods recommended by the National Ministry of Health,including the K-B method,the modified Hodge test and PCR amplification method.Results The K-B method showed that the antibacterial circle diameters of 50 strains to imipenem were less than or equal to 10mm.The modified Hodge test indicated that 50 strains were negative for carbapenemase.The agarose gel electrophoresis of the amplified products by PCR did not find the target gene bands.Conclusions The NDM-1 enzyme-producing superbugs were not prevalent in the Second Xiangya Hospital of Central South University.