复元胶囊对兔膝骨关节炎滑膜IL-1β、MMP-13及iNOS表达的影响

目的:通过观察复元胶囊对实验性兔膝骨关节炎（Osteoarthritis,OA）滑膜组织形态学变化的影响,以及复元胶囊对滑膜白细胞介素-1β（Interleukin-1β）、基质金属蛋白酶-13（Matrixmetalloproteinase-13）及诱导型一氧化氮合酶（Inducednitricoxidesynthase）表达的影响,探讨具有补益肝肾、益气活血作用的复元胶囊对实验性兔膝OA的治疗作用及其机理。方法:60只新西兰兔,随机分为复元胶囊低、中、高剂量组、模型组和正常组,用改良Hulth法制造兔膝OA模型,中药组给予复元胶囊灌胃,模型组给予生理盐水灌胃,连续分别用药4、8周和12周。HE染色光镜观察滑膜组织形态并评分,透射电镜观察滑膜组织超微结构,免疫组化检测滑膜IL-1β、MMP-13及iNOS的表达。实验后对滑膜评分和3个指标（IL-1β、MMP-13、iNOS）进行前后比较和组间比较。结果:复元胶囊低、中、高剂量组3个时间段的滑膜炎症明显小于同期模型组（P<0.05）,这3个治疗组3个时间段的滑膜IL-1β、MMP-13及iNOS的表达显著低于同期模型组（P<0.05）,中、高剂量组的差异非常显著（P<0.01）。结论:复元胶囊可明显改善实验性兔膝OA滑膜组织形态学的病理改变,显著抑制实验性兔膝OA滑膜组织的IL-1β、MMP-13和iNOS的表达。复元胶囊可改善实验性兔膝OA滑膜组织形态学的病理改变的作用与其抑制滑膜组织IL-1β、MMP-13和iNOS的表达密切相关。

Effects of Fuyuan capsule on expression of IL-1β,MMP-13 and iNOS in synovium in experimental osteoarthritis of rabbits

Objective:To observe the effects of fuyuan capsule on change of morphology of synovium,expression of IL-1β、MMP-13 and iNOS in synovium in experimental osteoarthritis of rabbits,and investigate the mechanism about the reparation and treatment of fuyuan capsule.Methods:Sixty New Zealand rabbits were divided into five groups randomly:normol group,model contrast group,low dose group, middle dose group and high dose group of fuyuan capsule.OA model was induced in rabbits by improved Hulth’S method.Three Chinese herb groups were treated with fuyuan capsule(0.727,1.453,4.795 g(/kg·d)),continuously for 4,8 weeks and 12 weeks,model contrast group was treated with 0.9%NS,ig,at the same time.Synovium sections were analyzed by immunohistochemistry for the IL-1β,MMP-13 and iNOS,observation and measurement of the change of morphology of synovium by light microscopic and transmission electron microscopic.After the experiment,the differences of these indexes between Three Chinese herb groups and model contrast group were compared.Results:There are found significant differences about measurement of morphology of synovium between Three Chinese herb groups and model contrast group after the experiment(P<0.05),especially high and middle dosage of fuyuan capsule group(P<0.01).It is the same as the comparison of the expression of IL-1β,MMP-13 and iNOS.Conclusion:Fuyuan capsule can improve the pathological change of morphology of synovium,and decrease the expression of IL-1β,MMP-13 and iNOS in synovium in experimental osteoarthritis of rabbit.There was therapeutical effect on synovium in experimental osteoarthritis of rabbit.