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Isolation of microsatellite loci in the Amazon sailfin catfish Pterygoplichlhys pardalis (Castelneau, 1855) (Teleostei: Loricariidae) |
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| Authors: | Rosalina P. Pereira Carlos Henrique dos A. dos Santos Priscila Roberta M. Nascimento Gisele T. Clímaco Adna Cristina B. Sousa Tatiana Campos Alexandre Manoel K. Vergueiro-Júnior Maria N. Paula-Silva Vera Maria F. Almeida-Val |
| Affiliation: | 1. Laborat??rio de Ecofisiologia e Evolu??o Molecular (LEEM), Instituto Nacional de Pesquisas da Amaz?nia (INPA), Aleixo, Manaus, AM, 69060-001, Brazil 2. Universidade do Estado da Bahia, Paulo Afonso, BA, 48608-230, Brazil 3. Empresa Brasileira de Pesquisa Agropecu??ria, Rio Branco, AC, 69908-970, Brazil |
| Abstract: | Pterygoplichlhys pardalis is an important freshwater ornamental fish in the Amazon Basin. Studies involving populations of P. pardalis are of great importance for the conservation and management of this species. We developed nine microsatellite loci and applied them to investigate the genetic variation of 20 wild individuals from floodplain lakes of the Solim?es river. The number of alleles per locus ranged from 2 to 12, with an average of 6.6. The observed and expected heterozygosity values ranged from 0.400 to 0.923 (average 0.706) and from 0.358 to 0.895 (average 0.692), respectively. The value of f ranged from ?0.532 to 0.467 (average 0.032). One locus (Pp07) significantly deviated from Hardy?CWeinberg equilibrium after Bonferroni correction (P: (5?%)?P. pardalis. |
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