| 山东肿足蕨总三萜化合物含量测定 |
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| 引用本文： | 张华丽,朱军,高桂花.山东肿足蕨总三萜化合物含量测定[J].济宁医学院学报,2011,34(3):160-161,164. |
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| 作者姓名： | 张华丽 朱军 高桂花 |
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| 作者单位： | 济宁医学院药学院,山东,日照,276826 |
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| 基金项目： | 济宁医学院2008年青年科研基金 |
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| 摘 要： | 目的 建立山东肿足蕨药材总三萜化合物的含量测定方法.方法 以齐墩果酸为对照品,以5%香草醛-冰醋酸溶液,浓硫酸为显色系统,采用紫外分光光度法测定总三萜化合物含量.结果 齐墩果酸在4.6～73.6 μg/ml范围呈良好的线性关系(r=0.9984),测定样品回收率为100.6%,RSD为1.1%.结论 该法简便、快速、灵...
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| 关 键 词： | 山东肿足蕨 总三萜化合物 含量测定 紫外分光光度法 |
Determination of total triterpenes in Hypodematium sinense Iwatsuri |
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| ZHANG Hua-li,ZHU Jun,GAO Gui-hua.Determination of total triterpenes in Hypodematium sinense Iwatsuri[J].Jounal of Jinning Medical College,2011,34(3):160-161,164. |
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| Authors: | ZHANG Hua-li ZHU Jun GAO Gui-hua |
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| Institution: | ZHANG Hua-li,ZHU Jun,GAO Gui-hua(College of Pharmacy,Jining Medical University,Rizhao 276826,China) |
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| Abstract: | Objective To establish a simple and accurate method for determination of total triterpenes in Hypodematium sinense Iwatsuri.Methods With oleanolic acid as the standard the content of total triterpenes was measured by spectrophotometry with 5% vanillin-glacial acetic acid solution and sulfuric acid as developing agent and detected in Hypodematium sinense Iwatsuriand.Results The calibration curve of oleanolic acid had good linear relationship in the range of 4.6~73.6μg/ml（r=0.9984）,and the average recovery was 100.6% with RSD 1.1%.Conclusion The method is simple,easy and reproducible that could be used as determination of total triterpenes in Hypodematium sinense Iwatsuri. |
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| Keywords: | Hypodematium sinense Iwatsuki Total triterpenes Content determination UV |
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