| 弓形虫表面抗原P22、P30复合基因在原核细胞中表达的研究 |
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| 引用本文： | 古钦民,王伟,卞继峰,丛华,胡海燕,何深一,李瑛. 弓形虫表面抗原P22、P30复合基因在原核细胞中表达的研究[J]. 山东大学学报(医学版), 2002, 40(2): 123-124,127 |
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| 作者姓名： | 古钦民 王伟 卞继峰 丛华 胡海燕 何深一 李瑛 |
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| 作者单位： | 山东大学医学院,寄生虫学教研室;山东大学医学院,医学分子生物学实验中心 |
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| 基金项目： | 山东省卫生厅科研基金资助课题 ( 99CA1CAA10 ) |
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| 摘 要： | 目的：对含有P22,P30复合基因的重组菌进行IPTG诱导表达,进而检测复合基因的融合表达产物的免疫活性。方法：用IPTG对工程菌进行诱导表达,表达产物行SDS－PAGE蛋白凝胶电泳及Western-Blot免疫印记检测,结果：蛋白电泳结果显示,阳性重组菌比阴性菌在66．5KDa位置上明显多一条带,此条带可与P30抗体结构并使免疫印记显示阳性结果。结论：含有P22、P30基因片段的质粒组体经诱导表达出融合蛋白,其内的组分蛋白P30具有与其相应抗体结合的能力。
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| 关 键 词： | 弓形虫 基因 P30 P22 基因表达 |
| 文章编号： | 1671-7554(2002)02-0123-02 |
Expression of P30 and P22 compound gene of the major surface antigen of toxoplasma gondii |
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| GU Qin min,WANG Wei,BIAN Jin feng,et al. Expression of P30 and P22 compound gene of the major surface antigen of toxoplasma gondii[J]. Journal of Shandong University:Health Sciences, 2002, 40(2): 123-124,127 |
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| Authors: | GU Qin min WANG Wei BIAN Jin feng et al |
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| Abstract: | Objective: To induce the engineering bacteria E.coli JM109 containing the recombinant plasmid ,and check up the activity of the expression products. Methods:Expression of the engineering bacteria was induced by IPTG, and the expression products were identified by SDS PAGE and Western Blot analysis. Results: The positive recombinant bacteria had one more protein band at 66.5 Kda site compared with the negative control. Western Blot with p30 antibody showed there was a brown band at the same site. Conclusion: Expression of P22 P30 fusion protein was successfully induced by IPTG, and it can be combined by P30 antibody. |
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| Keywords: | Toxoplasma gondii Gene P30 gene P22 Gene expression |
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