前列腺素E1脂微球制剂对油酸引起的大鼠急性肺损伤的实验研究

目的 :了解前列腺素E1脂微球制剂 (lipo PGE1)和粉针剂 (PGE1)在油酸引起的大鼠急性肺损伤中的应用价值。方法 :随机将 40只Wistar大鼠分成 4组 :正常组 (A组 )、油酸模型组 (B组 )、PGE1组 (C组 )和lipo PGE1组 (D组 )。于实验 3h时分别测量每只大鼠的肺系数、肺湿重 /肺干重、肺含水率、血浆中的丙二醛(MDA)、红细胞中的过氧化物歧化酶 (SOD)、动脉血气和、肺泡动脉氧分压差 (A aDO2 ) ,并进行统计学分析。结果 :(1 )D组与B组比较 ,肺系数、肺湿重 /肺干重、肺含水率显著减小 (P <0 .0 5 ) ,血浆SOD含量、动脉血PaO2 显著增高 (P <0 .0 5 ) ,血浆MDA含量、A aDO2 显著降低 (P <0 .0 5 )。 (2 )C组与B组比较 ,肺系数、肺湿重 /肺干重、肺含水率显著减小 (P <0 .0 5 ) ,动脉血PaO2 显著提高 (P <0 .0 5 ) ,A aDO2 显著降低 (P <0 .0 5 )。(3 )C组与D组比较 ,前者血浆SOD含量显著增高 (P <0 .0 5 ) ,血浆MDA含量显著降低 (P <0 .0 5 ) ,更接近正常。结论 :前列腺素E1脂微球制对油酸引起的大鼠急性肺损伤有明显的治疗作用 ,在某些方面其效果优于粉针剂型PGE1。

Effects of Lipo-PGE1 on oleic acid-induced acute lung injury in rats

Objective:To observe the efficacy of Lipo PGE 1 and PGE 1 powder on oleic acid induced acute lung injury in rats. Methods: Forty Wistar rats were randomly divided into 4 groups:normal control group,oleic acid induced group,PGE 1 powder group and Lipo PGE 1 group. Lung index, wet /dry weight and water ratio, SOD, MDA, arterial blood gases were measured and statistically analyzed.Results:(1)Lung index, wet /dry weight and water ratio were significantly lower ( P <0.05); SOD and PaO 2 levels were significantly higher ( P <0.05); A aDO 2 and MDA levels were markedly lower( P <0.05)in Lipo PGE 1 group than those in oleic acid induced group.(2)Lung index, wet /dry weight and water ratio were significantly lower ( P <0.05); PaO 2 levels were significantly higher ( P <0.05);and A aDO 2 levels were markedly lower ( P <0.05) in PGE 1 powder group than those in oleic acid induced group; But SOD and MDA levels were not significantly different between the two groups( P >0.05).(3)SOD and MDA levels were significantly different between Lipo PGE 1 group and PGE 1 powder group and more approaching the normal level in Lipo PGE 1 group. Conclusion: Lipo PGE 1 is more effective in the treatment of oleic acid induced acute lung injury in rats than PGE 1 powder.