干细胞治疗下肢缺血性疾病疗效观察

目的 观察自体外周血干细胞移植治疗缺血性下肢血管病的疗效。方法 移植组：对45例自愿接受自体外周血干细胞的患者使用重组人粒细胞集落刺激因子450～600U／d，皮下注射，进行外周血干细胞动员，第6天用血细胞分离机采集干细胞，配制成一定浓度的细胞混悬液，对缺血肢体进行移植。对照组：对33例患者使用扩血管药物治疗，连续20d。术后定期行皮温测定、踝臂指数观察和经皮氧分压测定，进行评估。结果 移植组患者术后各项观察指标均有改善，其中皮温、经皮氧分压改善明显，与对照组比较差异有统计学意义（P〈0．05）。结论 自体外周血干细胞移植治疗缺血性下肢血管病是一种安全、有效的手段，可使部分患者避免截肢或降低截肢平面，改善生活质量。

EFFECT OF AUTOLOGOUS PERIPHERAL BLOOD STEM CELLS TRANSPLANTATION IN TREATING ISCHEMIC LOWER EXTREMITY DISORDERS

Objective To observe the effect of autologous peripheral blood stem cells(PBSC) transplantation in the treatment of ischemic lower extremity disorders.Methods Therapeutic group:forty-five patients received recombinant human granulocyte colonystimulating factor 450 to 600 U/d by hypodermic injection for 5 days to mobilize stem cells.On the 6th day,PBSC were collected by COBE 6.1 Spectra Version and were injected into ischemic lower extremity.Control group:33 patients were treated with dilating vessels drugs.After operation some indexes were evaluated.Results After operation,these indexes were improved.Skin temperature and TcpO_2 were improved obviously,being statistically significant difference(P<0.05).Conclusion Autologous PBSC transplantation might be a safe and effective method for treating lower extremity ischemic disorder.It could improve the quality of life of many patients as amputation of lower extremity of foot might be avoided or reduced.