雾化吸入肺表面活性物质对急性肺损伤大鼠血液氧合水平的影响

目的 探讨雾化吸入肺表面活性物质(PS)对油酸型急性肺损伤(ALI)大鼠血液氧合水平的影响.方法 43只SD大鼠随机分为假手术组、模型组、雾化生理盐水组和雾化PS组.颈外静脉注入油酸(0.2 ml/kg)复制ALI模型,约1 h后雾化生理盐水组和雾化PS组分别雾化吸入生理盐水和160 mg PS.实验过程中测定大鼠血气...

Effect of aerosolized pulmonary surfactant on blood oxygenation in rats with acute lung injury

Objective To investigate the effect of aerosolized pulmonary surfactant on the blood oxygenation in rats with acute lung injury. Methods Forty three rats were randomly divided into four groups: sham group (operated only, n=10), model group(n=10), air-NS group (inhaling aerosolized normal saline, n=10), and air-PS group (inhaling aerosolized 160 mg PS, n=13). ALI was induced by 20% of oleic acid through jugular vein.Arterial blood gas and breathing rate of all groups were determined during the experiment. Survival rate was determined after experiment. Results PS aerosolization led to a gradual increase of PaO2 and SaO2. PaO2 and SaO2 increased to 99.4mmHg and 98.2% respectively after four hours, which had significant difference with air-NS group (p<0.05). Inhalation of aerosolized PS significantly decreased the breathing rate within half an hour and caused a gradual decrease to 100 breaths/minute after four hours. The survival rate in air-PS group was 100%, which had significant difference with air-NS group (60%, P < 0.05). Conclusion Aerosolized PS through spontaneous breathing is an effective, simple, and safe method for administration of exogenous surfactant.