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| c-myc ASODN对半乳糖诱导的晶体上皮细胞中两种抗氧化酶活性的影响 | |
| 引用本文： | 范芳,葛正龙,曾小平,李海祥,李长福. c-myc ASODN对半乳糖诱导的晶体上皮细胞中两种抗氧化酶活性的影响[J]. 遵义医学院学报, 2003, 26(5): 419-421 |
| 作者姓名： | 范芳 葛正龙 曾小平 李海祥 李长福 |
| 作者单位： | 1. 遵义医学院,生化教研室 2. 遵义医学院附属医院,眼科,贵州,遵义,563003 |
| 基金项目： | 国家自然科学基金(NO39460077),贵州省科委基金(93-3074) |
| 摘 要： | 目的 探讨ASODN对半乳糖性白内障晶体上皮细胞(LEC)中抗氧化系统活力的影响。方法 采用组织培养方法，以半乳糖诱发兔晶状体形成白内障，以不同浓度c-myc ASODN导入LEC后检测LEC中GSH-Px、SOD活性。结果 在半乳糖性白内障形成过程中，LEC中GSH-Px、SOD活性明显降低，导入不同浓度c-myc ASODN后，LEC中GSH-Px、SOD活性明显增加。结论在半乳糖性白内障形成过程中，导入c-myc ASODN后能改善LEC的抗氧化能力。 |
| 关 键 词： | 反义c-myc寡核苷酸 半乳糖 白内障 抗氧化系统 上皮细胞 抗氧化酶 c-mycASODN |
| 文章编号： | 1000-2715(2003)05-0419-03 |
| 修稿时间： | 2003-07-11 |
The effect of antisense oligodeoxynucleotide c-myc on anti-oxidation enzyme in LEC induced by galactose |
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| FAN Fang ,GE Zheng-long ,ZENG Xiao-ping ,LI Hai-xiang ,LI Chang-fu. The effect of antisense oligodeoxynucleotide c-myc on anti-oxidation enzyme in LEC induced by galactose[J]. Acta Academiae Medicine Zunyi, 2003, 26(5): 419-421 | |
| Authors: | FAN Fang GE Zheng-long ZENG Xiao-ping LI Hai-xiang LI Chang-fu |
| Affiliation: | FAN Fang 1,GE Zheng-long 1,ZENG Xiao-ping 2,LI Hai-xiang 2,LI Chang-fu 1 |
| Abstract: | Objective To study the effect of antisense oligodeoxynucleotide c-myc (c-myc ASODN) on anti-oxidation system in LEC induced by galactose. Methods Tissue culture was used to study the cataractous lenses of rabbit induced by galactose. C-myc ASODN in different doses were introduced into LEC. The activities of GSH-Px and SOD were detected in the cataratous lenses and they were comparecd with that of the normal lenses. Results The activities of GSH-Px and SOD in LEC decreased significantly during the formation of cataract induced by galactose. The different doses of c-myc ASODN can increase the activities of GSH-Px and SOD in LEC. Conclusions C-myc ASODN can improve the capability of anti-oxidation in LEC during the formation of galactosous cataract. [ |
| Keywords: | c-myc antisense oligodeoxynucleotide(c-myc ASODN) galactose cataract anti-oxidation system. |
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