大豆异黄酮与大豆皂甙抗辐射作用的实验研究

目的 探讨大豆异黄酮和大豆皂甙对γ射线辐射损伤的防护作用。方法 实验分三批,清洁级雄性昆明小鼠,每批按体重随机分为假照射组、照射模型组、大豆异黄酮组和大豆皂甙组。各受试物组小鼠经口灌胃各组受试物,连续30d后三批小鼠除假照射组外其余剂量组用⁶⁰Co-γ射线照射剂量分别为3.0Gy、5.0Gy、7.0Gy进行一次全身照射,照射后仍然给予受试物。分别检测外周血白细胞数、骨髓有核细胞数和骨髓细胞微核率以及红细胞与肝脏超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果 给予大豆异黄酮和大豆皂甙的小鼠在照射后第3天和第14天外周血白细胞数、骨髓有核细胞数、红细胞和肝脏SOD活力均高于照射模型组(P<0.05),骨髓微核率和血浆MDA水平低于照射模型组(P<0.05)。结论 大豆异黄酮和大豆皂甙均能提高受照射小鼠的抗氧化能力,在一定程度上减缓由辐射引起的外周血白细胞和骨髓有核细胞数下降,减少微核的产生,对γ射线损伤具有良好的防护作用。

Study on the Anti-Radiation Effect of Soyisoflavone and Soyasaponins

Objective To investigate the anti-γ radiation effect of soyisoflavone and soyasaponins.Methods Three batches in the experiment In every batch.clean rank of Kunming male mice were randomly divided into four groups:normal control group, radiated group, soyisoflavone group and soyasaponins group according to their weight.The mice in soyisoflavone group and soyasaponins group were fed with soyisoflavone and soyasaponins for 30 days successively, then the mice were irradiated by ⁶⁰Coγ-rays in the whole body except those in the normal control group.After radiation, these two groups were fed equally as before.WBC contents, the Granulocytes and the Micronucleated polychromatic erythrocytes in bone-marrow, SOD activities and MDA contents in the blood and liver were measured.Results On the third and the fourteenth day after radiation, the number of WBC in the blood and the number of the Granulocytes in bone-marrow, the activities of SOD in the blood and liver in soyisoflavone group and soyasaponins group were significantly higher than that of radiated group (P<0.05).The number of the Micronucleated polychromatic erythrocytes in bone-marrow and MDA contents in the blood were significantly lower than that of radiated group (P<0.05).Conclusion Soyisoflavone and soyasaponins could prevent the WBC in distal blood and the Granulocytes in bone-marrow decreasing in number after radiation, restrain the production of the Micronucleated polychromatic erythrocytes, improve the antioxidant ability of radiated mice, and release the irradiation injury from γ-rays.