江苏省脊髓灰质炎实验室细胞系敏感性实验方法的建立和监测

目的评价江苏省疾病预防控制中心脊髓灰质炎（脊灰）实验室所用细胞系对脊灰病毒的敏感性，制备江苏省脊灰实验室的标准毒株（QC）。方法采用96孔微量培养板滴定法。结果江苏省脊灰实验QC3次独立的细胞敏感性实验结果的滴度波动为±0．5 log 10CCID50，同时用中国疾病预防控制中心病毒病预防控制所国家脊灰实验室提供的已知滴度的Sabin参考株（China Sabin Test Reference Strain；CSTRS）做平行对照，CSTRS株3次滴度结果与其本身提供的参考值相比较，其滴度波动也均为±0．5细10CCID50。结论江苏省脊灰实验室QC结果符合实验要求，脊灰实验室所用细胞系对脊灰病毒的敏感性未下降。是敏感、有效的。

Establishment and Supervision of Experiment of Cell Sensitivity Test in Polio Laboratory of Jiangsu

bstract OBJECTIVE To evaluate the sensitivity of cell lines to poliovirus used in Jiangsu Provincial Polio Laboratory （JSPPL） and to prepare standard strain under the Quality Control （QC） of JSPPL. METHODS 96-microwells plate＇s culture and titration was used. RESULTS In the QC of JSPPL, the fluctuation of titre was ±0.5 log 10CCID50 in 3 independent cellular sensitivity tests. At the same time, China Sabin Test Reference Strain （CSTRS） provided by the National Polio Laboratory in viral disease control and prevention station in the National CDC was used as parallel contrast. The fluctuation of titre were also ±0.5 6910CCID50 in 3 independent cellular sensitivity test comparing with its own standard provided by CSTRS. CONCLUSIONS The result of QC in JSPPL was in accordance with experimental requirement. And the sensitivity of the cell line to poliovirus used in JSPPL was not dropping, which was sensitive and elective.