bFGF对体外培养的兔软骨细胞的胶原合成和稳定细胞表型的作用

为探寻软骨损伤修复的理论依据 ,取新西兰幼兔关节软骨组织 ,经消化后将软骨细胞分别接种在含有bFGF和缺乏bFGF的培养液中进行培养 ,测软骨细胞对 [3H] 脯氨酸的摄取量 ,观察载玻片上单层细胞经Vangieson染色后的胶原形态和电镜下胶原的形态 ,观察在细胞汇合生长情况下 ,bFGF对软骨细胞表型表达的影响。结果 :在含有bFGF和缺乏bFGF培养基中生长的软骨细胞对 [3H] 脯氨酸的摄取量的对数值有明显差异 (P <0 .0 5) ;电镜下可见加入bFGF培养出的软骨细胞分泌的胶原比未加bFGF培养出的软骨细胞分泌的胶原排列有序 ;加入bFGF后汇合生长的细胞表型无变化 ,而缺乏bFGF软骨细胞发生成纤维细胞样变。提示 :bFGF可促进软骨细胞合成胶原 ,对维持汇合生长的软骨细胞正常表型有重要作用。

Effects of bFGF onCollagen Synthesis and Stab ilization of Phenotypes of Cultured Rabbit ArticularChondrocyte in Vitro

The purpose of this study is to investigate effect of bFGF on rabbit articular chondrocyte culture in vitro. Chondrocytes were isolated from articular artilage of New Zealand rabbit of 1～3 d. Chondrocytes were seeded in 10 mL 1640 medium supplemented with 2% fetal calf serum(FCS)in the presence or absence of bFGF. Some slices were placed in culture flask before seeding chondrocytes. After 3 d in culture, cells were exposed to ^[3H]-proline for 24 h, then the rate of incorporation of ^[3H]-proline was measured and morphological appearance of collagen of chondrocytes monolayers was obsrved under electron microscope and light microscope. When chondrocyte was cultured for 2 weeks at confluence, chondrocyte was kept to cluture in the presence and absence of bFGF for 1 week, then morphological appearance of collagen was defected. The amount of incorporation of ^[3H]-proline was greater in chondrocyle cluture grown in the presence of bFGF than that in the absence of bFGF(P<0.05). In the presence of bFGF, configuration of collagenous fibersis appearced to be in a better order under electron microscope. Chondrocytes grown in absence of bFGF assumed gradual fibroblast configuration after confluent culture, but chondrocytes were Strikingly similar to normal chondrocytes in the presence of bFGF. It can be concluded that bFGF exerts a strong effect on stabilization of phenotypes of cultured chondrocytes and can stimulate the collagen synthesis.