| survivin 反义核酸诱导HeLa 细胞凋亡的实验 |
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| 引用本文： | 尹青,黄浩. survivin 反义核酸诱导HeLa 细胞凋亡的实验[J]. 肿瘤防治研究, 2008, 35(11): 791-792 |
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| 作者姓名： | 尹青 黄浩 |
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| 作者单位： | 1. 华中科技大学附属梨园医院妇产科,武汉,430077
2. 武汉第一医院实验中心 |
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| 摘 要： | 目的 探讨survivin 反义核酸诱导HeLa 细胞发生凋亡的可能性,揭示该凋亡发生与Bcl22 和 Bax 之间的关系。方法 采用TUNEL 染色法,研究survivin 反义核酸与HeLa 细胞凋亡的关系;通过免 疫组织化学法检测凋亡相关基因Bcl22 和Bax 的表达。结果 survivin 反义核酸在体外能诱导HeLa 细 胞凋亡,下调Bcl22 的表达,增强Bax 的表达。结论 诱导HeLa 细胞凋亡是survivin 反义核酸抗HeLa 作用的机制之一,survivin 反义核酸可能通过下调Bcl22 的表达,增强Bax 的表达,诱导HeLa 细胞发生 凋亡。
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| 关 键 词： | 反义核酸 宫颈癌 HeLa 细胞 凋亡 |
| 收稿时间： | 2007-09-18 |
| 修稿时间： | 2008-06-27 |
Apoptosis in HeLa Cells Induced by Antisense Oligodeoxynucleotide of Human survivin Gene |
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| YIN Qing,HUANG Hao. Apoptosis in HeLa Cells Induced by Antisense Oligodeoxynucleotide of Human survivin Gene[J]. Cancer Research on Prevention and Treatment, 2008, 35(11): 791-792 |
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| Authors: | YIN Qing HUANG Hao |
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| Affiliation: | YIN Qing 1,HUANG Hao2 1.Department of Obstetrics & Gynecology,Liyuan Hospital,Huazhong University of Science , Technology,Wuhan 430030,China,2.Center of Experimental Medicine,Wuhan First Hospital |
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| Abstract: | Objective To investigate the apoptosis in HeLa cell induced by antisense oligodeoxynucleotide of human survivin gene,and the relation between this apoptosis and expression of Bcl-2 and Bax.Methods In vitro experiments,TUNEL staining method was used to detect the apoptosis status of cervical cancer HeLa cell line treated by antisense oligodeoxynucleotide of survivin.Immunohistochemical staining was used to detect the expression of apoptosis-relation gene Bcl-2 and Bax.Results Antisense oligodeoxy nucleotide ... |
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| Keywords: | Antisense oligonucleotides Cervical cancer HeLa cell line Apoptosis |
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