Electron microscope studies of motile stages of malaria parasites. V. Exflagellation in Plasmodium, Hepatocystis and Leucocytozoon |
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| Authors: | P C Garnham R G Bird J R Baker |
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| Affiliation: | 1. Department of Pathology, Albert Einstein College of Medicine, Bronx, NY 10461, USA;2. Department of Microbiology, Immunology and Molecular Genetics, University of California Los Angeles, Los Angeles, CA 90095-1489, USA;3. Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA;4. Department of Developmental & Molecular Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA;5. Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA;6. Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA;3. Dipartimento di Malattie Infettive, Parassitarie ed Immunomediate, Istituto Superiore di Sanità, Viale Regina Elena n.299, 00161 Roma, Italy;;4. School of Biomedical and Healthcare Sciences, Plymouth University, Drake Circus, Plymouth, Devon, UK;;5. Dipartimento Tecnologie e Salute, Istituto Superiore di Sanità, Viale Regina Elena n.299, 00161 Roma, Italy;;6. Department of Vector Biology, Max-Planck Institute for Infection Biology, Charitéplatz 1, 10117 Berlin, Germany;;3. Department of Biochemistry, Virginia Tech, Blacksburg, Virginia 24061;3. From the Trypanosome Cell Biology Unit, Institut Pasteur & CNRS URA2581,;4. Proteomics Platform, Institut Pasteur,;5. Structural Mass Spectrometry and Proteomics Unit, Institut Pasteur & CNRS UMR3528,;6. Imagopole Platform, Institut Pasteur, Paris, France;1. Bioanalytics, Institute of Biotechnology, Technische Universität Berlin, 13355 Berlin, Germany;2. Wellcome Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3BF, UK;1. Pathogen Molecular Biology Department, Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, Keppel Street, London WC1E 7HT, UK;2. School of Biomedical and Healthcare Sciences, Plymouth University, Drake Circus, Plymouth PL4 8AA, UK |
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| Abstract: | ![]()
Exflagellating microgametocytes and microgametes of Plasmodium berghei, P. cynomolgi, Hepatocystis kochi and Leucocytozoon marchouxi were examined in the electron microscope. The morphology was similar in all species.Sections through the exflagellating gametocytes revealed 3 zones: (a) a light outermost envelope (not always present) representing the degenerating erythrocyte; (b) a dense region corresponding to the cytoplasm of the parasite and containing Golgi apparatus, endoplasmic reticulum, mitochondria, pigment and developing flagella; and (c) the nucleus undergoing endomitotic division, the 8 daughter nuclei eventually moving to the point of emergence of the gametes.Shadow-cast replicas of microgametes showed elongated bodies without free flagella.Sections of microgametes revealed the presence of an internal flagellar axial filament complex extending throughout their length, an elongated nucleus and a pellicle. The complex was made up of the typical 9 + 2 filaments with 9 radial spokes.The fundamental difference between the (short) microgamete of the Eimeriidea with 2 free flagella and the (long) microgamete of malaria parasites with no free flagellum is pointed out. A diagrammatic scheme of development of the latter is given. |
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