| 京大戟和甘遂炮制前后大戟二烯醇的含量比较 |
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| 引用本文： | 邱韵萦,郁红礼,吴皓,陶文婷,朱法根.京大戟和甘遂炮制前后大戟二烯醇的含量比较[J].中国药房,2012(11):1031-1033. |
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| 作者姓名： | 邱韵萦 郁红礼 吴皓 陶文婷 朱法根 |
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| 作者单位： | [1]南京中医药大学药学院,南京210029 [2]国家教育部中药炮制规范化及标准化工程研究中心,南京210029 [3]国家中医药管理局中药炮制标准重点研究室,南京210029 [4]江苏省中药炮制重点实验室,南京210029 |
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| 摘 要： | 目的:建立有毒中药京大戟与甘遂生品、醋制品中大戟二烯醇的含量测定方法,并比较两者炮制前后的含量变化。方法:采用高效液相色谱法。色谱柱为Hypersil ODS(250mm×4.6mm,5μm),流动相为乙腈-水(98∶2),柱温为30℃,流速为1.0mL·min-1,检测波长为210nm。结果:大戟二烯醇进样量在0.42～6.23μg范围内与峰面积积分值呈良好线性关系(r=0.9998);京大戟和甘遂生品的平均加样回收率分别为99.28%和99.80%,RSD分别为1.69%和1.83%(n=9)。京大戟中大戟二烯醇的含量明显高于甘遂,且醋制不会对大戟二烯醇的含量造成显著影响。结论:本方法能准确测定京大戟和甘遂中大戟二烯醇的含量。
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| 关 键 词： | 京大戟 甘遂 大戟二烯醇 炮制 含量 高效液相色谱法 |
Comparison of the Contents of Euphol in Euphorbia pekinensis and E. kansui before and after Processing |
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| QIU Yun-ying,YU Hong-li,WU Hao,TAO Wen-ting,ZHU Fa-gen.Comparison of the Contents of Euphol in Euphorbia pekinensis and E. kansui before and after Processing[J].China Pharmacy,2012(11):1031-1033. |
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| Authors: | QIU Yun-ying YU Hong-li WU Hao TAO Wen-ting ZHU Fa-gen |
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| Institution: | (Pharmacology College, Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China) YU Hong-li, WU Hao(Engineering Center for Standardization of Chinese Medicine Processing, Ministry of Education, Nanjing 210029, China) YU Hong-li, WU Hao(Key Laboratory for Standardization of Chinese Medicine Processing, State Administration of TCM, Nanjing 210029, China) YU Hong-li, WU Hao(Jiangsu Key Laboratory for TCM Processing, Nanjing 210029, China) |
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| Abstract: | OBJECTIVE： To develop a method for the determination of euphol in unprocessed Euphorbia pekinensis and E. kansui and their vinegar-processed products, and to compare the changes of euphol before and after process. METHODS： HPLC method was adopted. Samples were analyzed on a Hypersil ODS（250 mm×4.6 mm, 5 μm） column with mobile phase consisted of acetonitrile-water （98：2） at a flow of 1.0 mL.min-1 and the detection wavelength was 210 nm. The temperature of column was 30 ℃. RESULTS： The linear range of euphol was 0.42-6.23 ug（r=0.999 8） and the average recovery of E. pekinensis and E. kansui was 99.28% and 99.80% with RSD of 1.69% and 1.83% （n=9）, respectively. The content of euphol in E. pekinensis was higher than in E. kansui significantly. Vinegar process had no significant effect on the content of euphol. CONCLUSION： The method was accurate for the determination of euphol in E. pekinensis and E. kansui. |
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| Keywords: | Euphorbia pekinensis Euphorbia kansui Euphol Process Content HPLC |
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