RP-HPLC法同时测定利明滴眼液中维生素C和维生素B_1的含量

目的:建立同时测定利明滴眼液中维生素C和维生素B1含量的方法。方法:采用反相高效液相色谱法。色谱柱为AlltimC18,流动相为甲醇-30 mmol.L-1磷酸二氢铵溶液(5∶95,pH 3.2),流速为0.6 mL.min-1,检测波长为246 nm,柱温为25℃。结果:维生素C和维生素B1的检测浓度线性范围分别为7.5～360、11.92～198.64μg.mL-(1r=0.999 6、0.999 8,n=5);最低检测限分别为7.5、11.9 ng;日内、日间RSD和重复性试验RSD均小于2.0%;平均回收率分别为102.85%、101.43%(n=3)。结论:该方法简单、灵敏、准确,能有效控制该制剂的质量。

Simultaneous Determination of Vitamin C and Vitamin B_1 in Liming Eye Drops by RP-HPLC

OBJECTIVE：To establish the method for the simultaneous determination of vitamin C and vitamin B1 in Liming eye drops.METHODS：RP-HPLC method was adopted.The separation was carried out on Alltim C18 column with mobile phase consisted of methanol-30 mmol·L-1 ammonium dihydrogen phosphate solution（5 ∶ 95,adjusted pH to 3.2） at the flow rate of 0.6 mL·min-1.The detection wavelength was set at 246 nm and column temperature was 25 ℃.RESULTS：The linear ranges of vitamin C and vitamin B1 were 7.5～360 μg·mL-1（r=0.999 6,n=5）,11.92～198.64 μg·mL-1（r=0.999 8,n=5）,respectively.The detection limits were 7.5 ng and 11.9 ng,respectively.The RSDs of intra-day and inter-day and reproducibility did not exceed 2.0%,respectively.The average recoveries were 102.85% and 101.43%（n=3）.CONCLUSION： The method is simple,sensitive,accurate and could be applied to the quality control of Liming eye drops.