|
|||||
|
|
| 重组人结缔组织生长因子对人成骨细胞膜型基质金属蛋白酶1及基质金属蛋白酶2表达的影响 | |
| 引用本文： | 隋国良,彭依群,何玉玲,翟木绪,廖二元. 重组人结缔组织生长因子对人成骨细胞膜型基质金属蛋白酶1及基质金属蛋白酶2表达的影响[J]. 中华内分泌代谢杂志, 2011, 27(3). DOI: 10.3760/cma.j.issn.1000-6699.2011.03.016 |
| 作者姓名： | 隋国良 彭依群 何玉玲 翟木绪 廖二元 |
| 作者单位： | 1. 山东省烟台市烟台山医院内分泌科 2. 中南大学湘雅二医院代谢内分泌研究所,长沙,410011 |
| 摘 要： | 使用重组人结缔组织生长因子(recombinant human connective tissue growth factor,rCTGF)干预体外培养的人成骨细胞,发现rCTGF可呈时间-剂量依赖性地促进人成骨细胞膜型基质金属蛋白酶1及基质金属蛋白酶2的表达,200 ng/ml CTGF作用24～48 h达最大效果.rCTGF可明显增强p38丝裂原活化的蛋白激酶(p38-MAPK)磷酸化,p38-MAPK阻断剂SB 23058可阻断rCTGF上调膜型基质金属蛋白酶1及基质金属蛋白酶2的效应. |
| 关 键 词： | 重组人结缔组织生长因子 成骨细胞 膜型基质金属蛋白酶-1 基质金属蛋白酶-2 |
Effect of recombinant human connective tissue growth factor on the expression of membrane type-1 matrix metalloproteinase and matrix metalloproteinase 2 in human osteoblasts |
|
| SUI Guo-liang,PENG Yi-qun,HE Yu-ling,ZHAI Mu-xu,LIAO Er-yuan. Effect of recombinant human connective tissue growth factor on the expression of membrane type-1 matrix metalloproteinase and matrix metalloproteinase 2 in human osteoblasts[J]. Chinese Journal of Endocrinology and Metabolism, 2011, 27(3). DOI: 10.3760/cma.j.issn.1000-6699.2011.03.016 | |
| Authors: | SUI Guo-liang PENG Yi-qun HE Yu-ling ZHAI Mu-xu LIAO Er-yuan |
| Abstract: | Human osteoblast was treated with recombinant human connective tissue growth factor (rCTGF). This experiment showed that rCTGF increased membrane type-1 matrix metalloproteinase and matrix metalloproteinase-2 protein expression in a dose- and time-depentent manner in human osteoblasts. rCTGF induced activation of p38 MAPK in human osteoblasts. p38 MAPK inhibitor SB23058 abrogated the effect of rCTGF on the expressions of membrane type-1 matrix metalloproteinase and matrix metalloproteinase-2 in human osteoblasts. |
| Keywords: | Recombinant human connective tissue growth factor Human osteoblast Membrane type-1 matrix metalloproteinase Matrix metalloproteinase-2 |
| 本文献已被 万方数据 等数据库收录！ | |