糖尿病大鼠胃排空功能与胃组织瘦素的关系

大量文献已证实瘦素可抑制胃排空，胃排空延迟是糖尿病常见的并发症。目的：探讨不同病期糖尿病胃排空延迟与胃组织瘦素的关系。方法：40只Wistar大鼠分为对照1周组（NC1组）、对照4周组（NC2组）、糖尿病病期1周组（DM1组）和糖尿病病期4周组（DM2组）。分别于注射链佐霉素或柠檬酸缓冲液1、4周后检测大鼠胃排空、胃组织瘦素和瘦索受体OB—RbmRNA的表达。结果：与NC1组相比，DM1组大鼠胃排空明显加快（P〈0．01），胃组织瘦素表达显著增加（P〈0．01），OB—RbmRNA的表达无显著差异。与NC2组相比，DM2组大鼠胃排空明显减缓（P〈0．01），瘦素表达显著降低（P〈0．01），OB—RbmRNA的表达无显著差异。结论：随着糖尿病病期的持续，大鼠胃排空由加速转为延迟．胃组织瘦素表达反馈性由增加转为减少。

Relationship between Gastric Emptying and Gastric Leptin in Diabetic Rats

Many studies have demonstrated that leptin can inhibit gastric emptying, which is a common complication of diabetes. Aims： To investigate the relationship between delayed gastric emptying and gastric leptin in different diabetic stages. Methods： Forty Wistar rats were divided into four groups： normal control 1-week group （NC1 group）, normal control 4-week group （NC2 group）, diabetes mellitus 1-week group （DMI group） and diabetes mellitus 4- week group （DM2 group）. One week and four weeks after injection of streptozotocin or citrate buffer solution, gastric emptying, expressions of gastric leptin and its receptor OB-Rb mRNA were measured. Results： Compared with NCI group, gastric emptying and expression of gastric leptin increased significantly in DM~ group （P〈0,01）, OB-Rb mRNA expression had no significant differences. Compared with NC2 group, gastric emptying and leptin expression in DM2 group decreased significantly （P〈0.01）, OB-Rb mRNA expression also had no significant differences. Conclusions： With the development of diabetic stages, gastric emptying turns from acceleration to delay, and expression of gastric leptin from increase to decrease because of the feedback effect.