牵张刺激对滑膜细胞炎性因子表达的影响

目的：观察体外牵张刺激对滑膜细胞NF-κB、COX-2和iNOS表达的影响,探讨牵张刺激在颞下颌关节紊乱病（TMD）发病机制中的作用。方法：采用美国Flexercell公司生产的细胞柔性基底加载装置FX4000T对体外培养的HIG-82细胞进行周期性牵张,使用Western Blot及荧光显微镜技术检测不同牵张刺激下滑膜细胞NF-κB、COX-2和i-NOS的表达变化。结果：机械牵张刺激诱导激活了HIG-82细胞中NF-κB、COX-2和iNOS的表达;NF-κB抑制剂可抑制机械牵张诱导的COX-2表达。结论：体外牵张刺激可诱导激活HIG-82细胞中NF-κB、COX-2和iNOS的表达;COX-2的表达增加依赖于NF-κB的激活。

Effects of mechanical stretch on proinflammatory cytokine expression in synovial cells

Objective：To elucidate and visualize the effects that mechanical stretch of synovial cells induces COX-2 and iNOS expression via,activation of NF-kB.Method：HIG-82 rabbit synovial cells were cyclically stretched by Flexercell flexible substrate loading device FX4000T in vitro.And examine the increase of NF-κB,COX-2,iNOS by using luciferase reporter assay and western blotting.Result：Mechanical stretch induces expression of COX-2 and iNOS in HIG-82 cells and activates NF-kB.The inhibitors of NF-jB activation suppress stretch-induced expression of COX-2.Conclusion：Mechanical stretch on synovial cells can induce activation of NF-κB,COX-2 and iNOS in vitro;COX-2 expression was dependented on NF-κB activation.