一例罕见AB亚型的分子生物学研究分析

目的 分析研究一名献血者ABO血型形成的分子生物学机制。方法 用血清学方法检测ABO和H抗原,应用聚合酶链式反应(polymerase chain reation,PCR)检测其基因型,再对ABO基因第6,7外显子进行直接测序。结果 该献血员血清学检测为AB亚型,PCR结果为A2/Bw12,直接测序结果为A205/Bw12。结论 该献血员血型是一例比较罕见的AB双侧亚型。为保障输血安全,在该献血员不含抗体的情况下,其血液可以输注给配血相合的AB型患者。

Study on the Molecular Mechanism of a Case of Rare AB Subtype

Abstract:Objective To explore the molecular mechanism ofa blood donor's ABO blood group.Methods The serological test was applied to test the ABO and H antigens of the blood donor.The polymerase chain reation(PCR)was applied to exam the ABO genotype,and the direct sequencing was applied to test the exon6 and exon7 sequence of the blood donor'sABO gene.Results The results of the serological test indicated that the donor's ABO blood was AB subtype.The A2/Bw12 genotype was acquired with PCR and the A205/Bw12 genotpye was acquired with the direct sequencing.Conclusion The donor's blood type is a rare AB subtype with both A and B are subtypes.