非小细胞肺癌患者血清miR-141及miR-224的表达及其临床意义

目的 探讨miR-141及miR-224在非小细胞肺癌(NSCLC)中的表达及其临床意义。方法 采用RT-PCR检测128例NSCLC患者,60例良性病变患者(良性组)和60例健康体检者(对照组)血清miR-141,miR-224及鳞状细胞癌相关抗原(SCC-Ag)表达水平,分析miR-141及miR-224表达水平与NSCLC临床病理特征的关系。应用ROC曲线评价miR-141,miR-224及SCC-Ag对NSCLC诊断的灵敏度和特异度,Pearson相关分析NSCLC患者血清miR-141与miR-224,SCC-Ag的相关性。结果 NSCLC组血清miR-141,miR-224及SCC-Ag表达水平均明显高于良性组和对照组[miR-141(2^-ΔΔCt):2.56±0.48 vs 1.08±0.24和1.02±0.21; miR-224(2^-ΔΔCt):3.94±0.82 vs 1.42±0.35和1.26±0.30; SCC-Ag(ng/ml):2.75±1.36 vs 0.64±0.47和0.52±0.24,F=8.719～11.573,均P<0.01]。在NSCLC患者中血清miR-141及miR-224表达水平与病理分期、病理分级及淋巴结转移相关(P<0.05)。ROC曲线分析显示,血清miR-141,miR-224及SCC-Ag(ng/ml)诊断NSCLC的最佳截值分别为1.84,2.85,2.03,三项联合诊断NSCLC的AUC(0.913)最大,其敏感度和特异度较好,分别为92.5%和82.7%。Pearson相关分析显示,血清miR-141与miR-224,SCC-Ag呈正相关(r=0.782,0.594,均P<0.01),血清miR-224与SCC-Ag呈正相关(r=0.594,P<0.01)。结论 血清miR-141及miR-224在NSCLC患者中明显上调,且与患者的临床病理特征相关,有望作为诊断NSCLC的新型生物标志物。

Expression of Serum miR-141 and miR-224 in Patientswith Non-Small Cell Lung Cancer and Its Clinical Significance

Abstract:Objective To investigate the expression of miR-141 and miR-224 in non-small cell lung cancer(NSCLC)and its clinical significance.Methods The levels of serum miR-141,miR-224 and squamous cell carcinoma associated antigen(SCC-Ag)were detected by RT-PCRin 128 patients with NSCLC,60 patients with benign lesions(benign group)and60 healthy subjects(control group).To analyze the relationship between the expression levels of miR-141 and miR-224 and the clinicopathological features ofNSCLC.The sensitivity and specificity of miR-141,miR-224 and SCC-Ag in thediagnosis of NSCLC were evaluated by ROC curve,and the correlation between serum miR-141 and miR-224,SCC-Ag were analyzed by Pearson correlation analysis in NSCLC patients.Results The levels of serum miR-141,miR-224 and SCC-Ag in NSCLC group were significantly higher than those in the benign group and the control group[miR-141(2^-ΔΔCt):2.56±0.48 vs1.08±0.24 and 1.02±0.21,miR-224(2^-ΔΔCt):3.94±0.82 vs 1.42±0.35 and 1.26±0.30,SCC-Ag(ng/ml):2.75±1.36 vs 0.64±0.47 and0.52±0.24,all P<0.01].In patients with NSCLC,the levels of serum miR-141 and miR-224 were correlated with pathological stage,pathological grade and lymph node metastasis(P<0.05).ROC curve analysis showed that the optimal cut-off values of serum miR-141,miR-224 and SCC-Ag(ng/ml)for diagnosisof NSCLC were 1.84,2.85 and 2.03,respectively.The AUC(0.913)of the three combined diagnosis of NSCLC was the largest,and the sensitivity and specificity were better,with 92.5% and 82.7%,respectively.Pearson correlation analysis showed that serum miR-141 were positively correlated with miR-224 and SCC-Ag(r=0.782,0.594,all P<0.01),and serum miR-224 was positively correlated with SCC-Ag(r=0.594,P<0.01).Conclusion Serum miR-141 and miR-224 are up-regulated in patients with NSCLC and are associated with clinicopathological features,and may be a new biomarker forthe diagnosis of NSCLC.