血管内皮生长因子C及LYVE-1在肌肉瘤动物模型中的表达

目的建立横纹肌肉瘤模型,研究肌肉瘤淋巴道转移机制,为临床肌肉瘤治疗提供理论依据。方法取清洁级昆明系小鼠30只,用小鼠S180癌性腹水,在小鼠臀部肌层种植,建立肌纤维肉瘤的原位瘤种植模型,观察肿瘤的生长情况,1周后处死小鼠,切取瘤体,检测肿瘤体积、重量及开胸、腹观察肿瘤转移情况及不同时期肿瘤血管、淋巴管生长与肿瘤生长转移的关系。取肿瘤组织包埋制备切片,应用免疫组化、Western blot等方法检测血管内皮生长因子C(VEGF-C)蛋白质表达。应用淋巴管内皮透明质酸受体-1(LYVE-1)标记淋巴管,检测肌肉瘤组织中的淋巴管密度(LMVD)。结果横纹肌肉瘤组织中心区和边缘区均存在淋巴管,这预示着横纹肌肉瘤组织中存在淋巴管的增生和新生,其可能与VEGF-C的诱导密切相关。结论横纹肌肉瘤组织中VEGF-C的表达增高,预示VEGF-C与横纹肌肉瘤淋巴管新生密切相关,从而促进肿瘤的浸润和转移。

Expression of VEGF-C and LYVE-1 in animal models of myosarcoma

【Objective】 To establish rhabdomyosarcoma model for researching the mechanism of lymphatic and hematogenous metastasis in order to provide theoretical basis for clinical myosarcoma treatment.【Method】 30 mice were inoculated with malignant ascites of S180 mice in muscular layer of gluteal region to establish myofibrosarcoma orthotopic tumor model.The mice were sacrificed in the different time after 1 week and the volume and weight of tumors were detected.The condition of neoplasm metastasis and the relation of tumor vessel and the growth of lymphatic and tumor metastatic were observed after opening the chest and abdomen.the tissue of tumor was used for embedding and slicing.Immunohistochemisty and western blot were used to detect the expression of VEGF-C.LMVD in myosarcoma was calculated by using LYVE-1 as the specific marker of lymphatic endothelium.【Results】 There were many lymphatic vessels in centre and marginal zone of rhabdomyosarcoma tissue.It showed that rhabdomyosarcoma tissue had lymphatic hyperplasia and neogenesis.【Conclusion】 The positive expression of VEGF-C in rhabdomyosarcoma is related with lymphatic neogenesis.VEGF-C might play an important role in lymphatic metastasis of rhabdomyosarcoma.