| 反相高效液相色谱法测定阿霉素聚乳酸微球的含量 |
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| 引用本文： | 阎志飞,赵志娟,丁红,罗斌华. 反相高效液相色谱法测定阿霉素聚乳酸微球的含量[J]. 中国药物与临床, 2010, 10(7): 754-755 |
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| 作者姓名： | 阎志飞 赵志娟 丁红 罗斌华 |
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| 作者单位： | 1. 山西医科大学药学院药剂学教研室,太原,030001
2. 湖北咸宁学院药学院 |
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| 基金项目： | 山西医科大学青年基金 |
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| 摘 要： | 目的采用反相高效液相色谱法测定阿霉素聚乳酸微球中的含量。方法采用AgilentEclipseXDB-C18色谱柱(150mm×4.6mm,5μm),以乙腈-甲醇-水(4∶5∶1)为流动相,检测波长为233nm。结果阿霉素在5～50μg/ml与峰面积呈良好的线性关系(r=0.9994),方法平均回收率为99.92%,RSD=0.82%。结论反相高效液相色谱法测定阿霉素聚乳酸微球的含量简便易行,专属性强,灵敏度高,重复性好,结果准确。
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| 关 键 词： | 色谱,高效液相法 阿霉素 微球体 聚乳酸 |
HPLC determination of loading amount of adriamycin polylactic acid microspheres |
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| YAN Zhi-fei,ZHA Zhi-juan,DING Hong,LUO Bin-hua. HPLC determination of loading amount of adriamycin polylactic acid microspheres[J]. Chinese Remedies & Clinics, 2010, 10(7): 754-755 |
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| Authors: | YAN Zhi-fei ZHA Zhi-juan DING Hong LUO Bin-hua |
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| Affiliation: | . Department of Pharmaceutics, School of Pharmaceutial Science, Shanxi Medical U- niversity, Taiyuan 030001, China |
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| Abstract: | Objective To determine the loading amount of doxorubicin polylactic acid microspheres by RP- HPLC. Methods An RP-HPLC method was employed for determination using Agilent Eclipse XBD-C18 column （150 mm×4.6 mm, 5 μm） with a mobile phase consisted of acetonitrile-methanol-water （4∶5∶1）. The length of wavelength was 233 nm. Results Good linearity was achieved in the range of 5～50 μg/ml （r=0.9994）. The recovery was 99.92% with RSD of 0.82%. Conclusion This method appeared easy-to-use, specific and sensitive, reproducible and accurate. |
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| Keywords: | Chromatography high pressure liquid Adriamycin Microspheres Polylactic acid |
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