| X染色体耦联锌指蛋白在人脑胶质瘤干祖细胞中的表达及意义 |
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| 引用本文： | 苏作鹏,周幽心,陈雄辉,陈金明,陈三送,吴庭枫,陈桂林.X染色体耦联锌指蛋白在人脑胶质瘤干祖细胞中的表达及意义[J].江苏医药,2012,38(10):1121-1123,1112. |
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| 作者姓名： | 苏作鹏 周幽心 陈雄辉 陈金明 陈三送 吴庭枫 陈桂林 |
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| 作者单位： | 苏作鹏 (苏州大学附属第一医院神经外科, 江苏省,215006) ； 周幽心 (苏州大学附属第一医院神经外科, 江苏省,215006) ； 陈雄辉 (苏州大学附属第一医院神经外科, 江苏省,215006) ； 陈金明 (苏州大学附属第一医院神经外科, 江苏省,215006) ； 陈三送 (苏州大学附属第一医院神经外科, 江苏省,215006) ； 吴庭枫 (苏州大学附属第一医院神经外科, 江苏省,215006) ； 陈桂林 (苏州大学附属第一医院神经外科, 江苏省,215006) ； |
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| 基金项目： | 江苏省卫生厅基金,苏州市科技计划资助项目 |
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| 摘 要： | 目的探讨X染色体耦联锌指蛋白(Zfx)在人脑胶质瘤干祖细胞中的表达及意义。方法逆转录PCR方法检测人脑胶质瘤干祖细胞SU2及其分化后贴壁细胞中mRNA的表达;细胞免疫组化检测人脑胶质瘤干祖细胞SU2中Zfx、CD133、nestin的表达;然后在含有10%胎牛血清培养液中诱导SU2分化,并检测分化后的细胞中Zfx、S100、GFAP的表达。结果 Zfx在胶质瘤干祖细胞SU2及其分化后贴壁细胞中的mRNA均有表达。Zfx在人脑胶质瘤干祖细胞SU2中的蛋白水平表达高,但SU2经诱导分化后细胞中Zfx蛋白水平表达减弱。结论 Zfx在人脑胶质瘤干祖细胞中高表达,可能在人脑胶质瘤干祖细胞自我更新和分化抑制过程中起重要作用。
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| 关 键 词： | X染色体耦联锌指蛋白 胶质瘤干祖细胞 |
Expression and significance of X-linked zinc-finger protein in human glioma stem/progenitor cells |
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| Institution: | SU Zuopeng,ZHOU Youxin,CHEN Xionghui,et al.Department of Neurosurgery,Affiliated First Hospital,Soochow University,Suzhou 215006,CHINA |
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| Abstract: | Objective To investigate the expression and significance of X-linked zinc-finger protein(Zfx) in human glioma stem cells.Methods The expression of Zfx mRNA in glioma stem/progenitor cells SU2 and serum-induced cells was detected by RT-PCR.The expressions of Zfx,CD133,nestin were examined by immunofluorescence staining.SU2 was cultured in medium supplemented with 10% FBS and induced to differentiate,in which The expressions of Zfx,S100,GFAP were examined by immunofluorescence stainings.Results Zfx mRNA was expressed in human glioma stem/progenitor cells and its differentiated cells.The expression of Zfx protein level was higher in brain glioma stem/progenitor cells than that in the differentiated cells.Conclusion Zfx is over-expressed in glioma stem/progenitor cells,which may play an important role in the self-renewal of glioma stem/progenitor cells. |
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| Keywords: | X-linked zinc-finger protein Glioma stem/progenitor cells |
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