丙泊酚对BV-2小胶质细胞活化的影响

目的探讨丙泊酚对脂多糖(LPS)诱导的BV-2小胶质细胞活化的影响及其可能机制。方法体外培养小鼠BV-2小胶质细胞,随机分为LPS 1μg/ml组(A组)、丙泊酚30μM组(B组)、LPS 1μg/ml+丙泊酚30μM组(C组)和空白对照组(D组)。采用RT-PCR检测各组细胞中IL-1β和TNF-αmRNA表达量,Western blot检测总糖原合成酶激酶-3β(GSK-3β)和磷酸化GSK-3β(p-GSK-3β)的蛋白表达水平。结果 A、C组IL-1β、TNF-α及p-GSK-3表达量均较D组明显增加(P<0.05或P<0.01)。与A组相比,C组IL-1β和TNF-αmRNA表达量降低,而p-GSK-3β蛋白表达量增加(P<0.05)。结论丙泊酚30μM能在体外减轻LPS诱导的BV-2小胶质细胞释放IL-1β和TNF-α的水平,此作用可能与抑制GSK-3β活性有关。

Effect of propofol on lipopolysaccharides-induced activation of BV-2 microglia cells

Objective To investigate the effect and possible mechanism of propofol on lipopolysaccharides(LPS)-induced activation of BV-2 microglia cells.Methods BV-2 microglia cells were cultured in vitro and randomly divided into four groups of A(treated with LPS 1 μg/ml),B(treated with propofol 30 μM),C(treated with LPS 1 μg/ml plus propofol 30 μM) and D(blank control).The mRNA expressions of IL-1β and TNF-α were detected by RT-PCR.The protein expressions of total glycogen synthase kinase-3β(GSK-3β) and phosphorylation of GSK-3β(p-GSK-3β) were analyzed by Western blot.Results The expressions of IL-1β,TNF-α and p-GSK-3β in groups of A and C were significantly higher than those in group D(P<0.05 or P<0.01).Compared with group A,the mRNA expressions of IL-1β and TNF-α were decreased,while protein expression of p-GSK-3β was increased in group C(P<0.05).Conclusion Propofol 30 μM down-regulates LPS-induced expressions of IL-1β and TNF-α in BV-2 microglia cells in vitro,which is possibly related with inhibiting the activation of GSK-3β.