太行山区柴胡中总皂苷及柴胡皂苷a、d的含量测定

目的建立柴胡药材中总皂苷及柴胡皂苷a、d的含量测定方法,并对太行山区的柴胡样品进行含量测定。方法采用紫外分光光度法,以柴胡皂苷d为对照品,在波长545 nm处对样品中的总皂苷进行含量测定;采用HPLC法,以C18色谱柱（250 mm×4.6 mm,5μm）、乙腈-水为流动相,流速为1.0 mL/min,检测波长为210 nm,测定样品中柴胡皂苷a、d的含量。结果柴胡总皂苷在0.032 56～0.162 80 mg、柴胡皂苷a在0.258～2.580μg、柴胡皂苷d在0.238～2.380μg范围内呈良好的线性关系,r分别为0.996 9、0.999 6、0.999 7;平均回收率：柴胡总皂苷为99.73%,柴胡皂苷a为100.40%,柴胡皂苷d为99.70%;RSD：柴胡总皂苷为2.07%,柴胡皂苷a为1.72%,柴胡皂苷d为1.50%。结论本研究所建立的紫外分光光度法测定柴胡总皂苷及高效液相法测定柴胡皂苷a、d含量的方法,简单、易行、快速,结果准确可靠,适用于柴胡中柴胡总皂苷及柴胡皂苷a、d的含量测定,并为在安全剂量范围内正确使用柴胡提供依据。

Determination of the content of saikosaponins and saikosaponin a,d of Radix Bupleuri from Taihang Mountain area

Objective To establish the method for determination of the content of saikosaponins,saikosaponin a and d in Bupleurum Chinense DC,and carries on determination of the content of the samples of saikosaponins,saikosaponin a and d from Taihang Mountain area.Methods Saikosaponin d was used as the chemical reference substance determine the content of saikosaponins by UV-Visible Spectrophotometry.The absorbency was measured at 545 nm.The content of saikosaponin a and d in Bupleurum Chinense DC was determined by HPLC.The samples were separated by C 18 column（250 mm×4.6 mm,5 μm）.The mobile phase was acetonitrile-water.The flow was 1.0 mL/min.The detector wavelength was 210 nm.Results There was good linearity in the range of 0.032 56-0.162 80 mg（r = 0.996 9） for saikosaponins,in the range of 0.258-2.580 μg（r = 0.999 6） for saikosaponin a,in the range of 0.238-2.380 μg（r = 0.999 7） for saikosaponin d;the average recovery of saikosaponins,saikosaponin a and d were 99.73%,100.40% and 99.70%,and RSD were 2.07%,1.72% and 1.50%.Conclusion The methods for determination of the content of saikosaponins by UV-visible spectrophotometry and saikosaponin a and d by HPLC were proved to be simple,easy and fast to perform,and the results obtained were reliable and suitable.So,it can be used for the quantitative determination of saikosaponins,saikosaponin a and d in Bupleurum Chinense DC.,and to provide the basis for correctly using Bupleurum Chinense DC.in the safe dose scope.