基于DNA条形码技术的维吾尔药材桑葚基原鉴定研究

该研究通过分析多途径来源的新疆桑属植物及药材样本的ITS2,psbA-trnH序列,为药材分子鉴定提供依据。以51个新疆桑属植物及药材为样本,对其ITS2,psbA-trnH序列进行PCR扩增和测序,用MEGA 6.0计算其种内、种间Kimura 2-parameter(K2P)距离,分析变异位点,并构建NJ鉴别树。ITS2序列分析结果显示,桑Morus alba、鞑靼桑M.alba var.tatarica、黑桑M.nigra种内无变异;桑与鞑靼桑种间无变异;桑与黑桑种间存在13个变异位点,种间平均KP2遗传距离为0.04;桑与药材样本间无信息变异位点,NJ鉴别树可将桑及鞑靼桑与黑桑区分。psbA-trnH序列分析结果显示,桑与黑桑种内各有1个变异位点,3种植物种间存在插入/缺失变异,可相互区分;种间变异与药材样本内变异一致。因此,ITS2序列可将来源于桑、鞑靼桑的药材样本与黑桑区分,psbA-trnH序列可将来源于三者的药材样本区分,为维吾尔药材真伪鉴别及市场监管提供依据。

Identification of origin plant of Uygur medicine mulberry based on DNA barcode

To provide molecular evidence for medical material identification, we analyzed the nucleotide sequence of ITS2, psbA-trnH gene in Morus genus plants and commercial products which were obtained from different places in Xinjiang. The sequence of ITS2 and psbA-trnH in fifty-one samples were amplified and sequenced, MEGA 6.0 was used to analyze the intra- and interspecific K-2P distances, neighbor-joining (NJ) tree was used to constructing clustering tree. ITS2 sequence analyzed results showed that there is no intra-specific variation among Morus alba, M. alba var. tatarica and M. nigra, but 13 variations sites were exist between M. alba and M. nigra and their inter-specific K-2P distances was 0.04, which indicated that there had significant variation in them. We didn''t find informative variation sites between Morus genus plants and commercial products, and we also found that M. nigra can be distinguished from other two species by NJ Tree. PsbA-trnH analysis results showed there was only one variation site between M. alba and M. nigra, but insertion or deletion variation were remarkable evidence among M. alba, M. alba var. tatarica and M. Nigra. Inter-specific variation was accordance with intra-specific variation of commercial products. So ITS2 and psbA-trnH gene were important marker for M. alba, M. alba var. tatarica and M. nigra identification. This study provided important evidence for Uygur medicine identification and market supervision.