自贡市食品中单核细胞增生李斯特菌污染状况的调查及药敏结果分析

目的]调查自贡市食品中单核细胞增生李斯特菌(LM)污染状况及药敏情况。方法]检测按国标GB/T4789.30-2003程序进行;药敏试验采用Kirby-Bauer法。结果]2005~2007年于253份食品中共检出LM21株;总污染率为8.3%(21/253)。样品中生畜肉、生禽肉、熟肉及海水产品的污染率分别为18.3%(15/82)、3.4%(2/59)、4.4%(3/68)、2.3%(1/44)。对15株LM进行了18种抗生素的药敏试验,其中对庆大霉素、链霉素、左氟沙星、先锋V、四环素、万古霉素、青霉素G、红霉素、复方新诺明等全部敏感;对多粘菌素B、氯霉素等部分耐药。结论]用CHROMAGAR显色培养基分离LM,是一种理想途径,可明显提高检测效率。进行食品中LM污染卫生学调查,掌握该菌在主要食品中的污染分布特点及敏感药物,为预防与治疗因LM引发食源性疾病或食物中毒的潜在危险及食品安全管理提供科学依据。

INVESTIGATION ON POLLUTION STATUS OF LISTERIA MONOCYTOGENES IN FOOD AND DRUG SENSITIVITY IN ZIGONG

Objective] To investigate pollution status of Listeria monocytogenes (LM) in food and drug sensitivity. Methods] Detected samples according to GB / T4789.30-2003 and drug sensitivity with Kirby-Bauer method. Results] From 2005 to 2007, 21 strains of LM were found in 253 food samples, the total contamination rate was 10.2% (21 / 205) .The con-tamination rates were 18.3% (15 / 82) of fresh meat, 3.4% (2 / 59) of fresh birds meat, 4.4% (3 / 68) of cooked meet prod-ucts and 2.3% (1 / 44) of sea food respectively. Susceptibility testing was taken for 15 strains of LM by 18 kinds of antibiotics, as a result, all the 15 strains of LM were sensitive to Gentamicin, Streptomycin, Levofloxacin, Pioneer adriamycin V, Tetra-cycline, Vancomycin, Penicillin G, Erythromycin, Sulfamethoxazole .Some strains of LM were found antibiotic-resistant to Polymyxin B, Chloramphenicol, and so on. Conclusion] Using CHROMAGAR chromogenic medium is a ideal method to separate LM and the detection efficiency is markedly improved. Hygiene investigation of LM pollution in food could make known polluting distribution characters and sensitive drugs of the bacteria, and is helpful for prevention and treatment of foodborne dis-eases or food poisoning caused by LM. It also could provide scientific evidences for food safety management.