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紫萁贯众中多糖的含量测定
引用本文:厉博文,张东,杨岚,傅梅红,方婧. 紫萁贯众中多糖的含量测定[J]. 中国实验方剂学杂志, 2010, 16(10): 41-43
作者姓名:厉博文  张东  杨岚  傅梅红  方婧
作者单位:中国中医科学院中药研究所,北京,100700
基金项目:《中国药典》2010版标准研究 
摘    要:
目的:建立紫萁贯众中总多糖的含量测定方法,比较不同产地和采收期紫萁贯众多糖含量。方法:采用硫酸-苯酚法显色,分光光度法在490nm处测定。结果:12个不同产地紫萁贯众多糖含量为1.22%~1.85%,同产地不同采收期紫萁贯众多糖含量为0.91%~1.37%。结论:紫萁贯众多糖含量因产地和采收期变化而不同。

关 键 词:紫萁贯众  总多糖  硫酸-苯酚法

Determination of Polysaccharides from Rhizoma of Osmunda japonica
LI Bo-wen,ZHANG Dong,YANG Lan,FU Mei-hong and FANG Jing. Determination of Polysaccharides from Rhizoma of Osmunda japonica[J]. China Journal of Experimental Traditional Medical Formulae, 2010, 16(10): 41-43
Authors:LI Bo-wen  ZHANG Dong  YANG Lan  FU Mei-hong  FANG Jing
Affiliation:Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
Abstract:
To establish the method for determination of polysaccharide from Rhizoma of Osmunda japonica and the content of polysaccharide from Rhizoma of O. japonica in different locations and different harvest times was compared. Method: Sulfuric acid-phenol method was used and the sugar content was determined by the spectrophotometer at 490 nm. Result: The content of polysaccharide from Rhizoma of O. japonica in different locations was 1.22%-1.85% and in different harvest times was 0.91%-1.37%. Conclusion: The different locations and different harvest times would affect the content of polysaccharide from Rhizoma of O. japonica.
Keywords:Osmunda japonica  polysaccharide  sulfuric acid-phenol method
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