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| 地西他滨联合阿霉素逆转白血病K562细胞株多药耐药作用观察 | |
| 引用本文： | 李静怡,邓琦,刘鹏江,赵明峰,李玉明,王学谦. 地西他滨联合阿霉素逆转白血病K562细胞株多药耐药作用观察[J]. 山东医药, 2010, 50(17): 19-21 |
| 作者姓名： | 李静怡 邓琦 刘鹏江 赵明峰 李玉明 王学谦 |
| 作者单位： | 1. 天津医科大学研究生院,天津,300070;天津市第一中心医院 2. 天津市第一中心医院 |
| 基金项目： | 天津市卫生局科技项目 |
| 摘 要： | 目的观察地西他滨（DCA）联合阿霉素（ADR）对白血病K562细胞株多药耐药的逆转作用,探讨其治疗白血病的可行性。方法以不同浓度DCA＋ADR处理K562/ADR细胞,用台盼蓝法观察药物对细胞生长曲线的影响,MTT法检测不同浓度DCA与ADR对K562/ADR细胞的杀伤活性,流式细胞技术检测细胞膜P糖蛋白及细胞内ADR浓度。结果 DCA＋ADR对白血病K562细胞株多药耐药有明显逆转作用,可降低细胞膜P糖蛋白表达,提高K562耐药细胞株对ADR的敏感性。结论 DCA联合ADR可逆转白血病K562细胞株多药耐药性。 |
| 关 键 词： | 白血病 地西他滨 阿霉素 K562/ADR细胞 |
Reversal effect of decitabine and adriamycin on the multidrug resistance of leukemia cell strain K562 |
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| LI Jing-yi,DENG Qi,LIU Peng-jiang,ZHAO Ming-feng,LI Yu-ming,WANG Xue-qian. Reversal effect of decitabine and adriamycin on the multidrug resistance of leukemia cell strain K562[J]. Shandong Medical Journal, 2010, 50(17): 19-21 | |
| Authors: | LI Jing-yi DENG Qi LIU Peng-jiang ZHAO Ming-feng LI Yu-ming WANG Xue-qian |
| Affiliation: | LI Jing-yi1,DENG Qi,LIU Peng-jiang,ZHAO Ming-feng,LI Yu-ming,WANG Xue-qian(1 Graduate School of Tianjin Medical University,Tianjin 300070,P.R.China) |
| Abstract: | Objective To observe the reversal effect of decitabine and adriamycin(ADR) on the multidrug resistance of leukemia cell strain K562 and to explore its feasibility to treat leukemia.Methods K562/ADR cells were treated with different concentrations of decitabine and ADR.The impact of cell growth curve was observed by Trypan blue staining.Killer acticity of K562/ADR cell with different concentrations of decitabine or ADR was determined by MTT assay.The concentrations of P-glycoprotein in cell membrane and of A... |
| Keywords: | leukemia decitabine adriamycin K562/ADR cells |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |