低分子肝素脂质体的研究

 目的研究以Natipide Ⅱ空白脂质体包封低分子肝素形成脂质体的性质，为其制剂的开发提供参考。方法以超滤法测定脂质体包封率和不同温度下的渗漏率，以透射电镜观察其形态，以表面电位粒径仪测定脂质体颗粒表面电荷及zeta电位。结果表观重量包封率43.18%，颗粒为圆形或椭圆形，平均粒径为200 nm，平均zeta电位为-66.45 mV，在4，15，25℃放置3个月后渗漏率分别为10.26%，16.58%和25.54%。结论Natipide Ⅱ空白脂质体对低分子肝素具有较强的包封能力，形成的脂质体较为稳定，具有开发价值。

Study on liposomes of low molecular weight heparin

OBJECTIVE To study the characteristics of liposomes of LMWH encapsulated by proliposomes.METHODS Encapsulated ratio of liposomes and leaked ratio at different temperature were determined by ultra filtration,the shape of liposomes was observed under the electronic microscope and zeta potential of liposomal vesicle was determined with the instrument.RESULTS The apparent weight encapsulated ratio of liposomes was 43.18%.The vesicles were globular or elliptic.The mean diameter was about 200 nm.The mean zeta potential was -66.45mV,and the leaked ratio was respectively 10.26%,16.58%,and 25.54% at 4,15,25℃ after 3 months.CONCLUSION The proliposomes had well encapsulating ability for LMWH.The liposome system of LMWH was stable at low temperature and worthy further exploiting.