| Cryptophycin诱导肾癌细胞凋亡的研究 |
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| 引用本文： | 周鹤同 郭震华. Cryptophycin诱导肾癌细胞凋亡的研究[J]. 实用癌症杂志, 2000, 15(3): 235-237 |
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| 作者姓名： | 周鹤同 郭震华 |
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| 作者单位： | 南京江北人民医院(周鹤同!210048,邵竹平!210048,李东!210048),苏州医学院第一附属医院(郭震华!215006,严春寅!215006),解放军第81医院全军肿瘤研究中心(苏长青!210002) |
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| 摘 要： | 目的 探讨诱导肾癌细胞凋亡的新方法及其机制。方法 采用ＨＥ滩色、荧光染色、琼脂糖电池等方法,分别对Ｃｒｙｔｏｐｈｙｃｉｎ（ＣＰＰ）不同的药物浓度（０,１,５,１０,５０ｐｍｏｌ／Ｌ）和不同增减时间段（４～２４ｈ）所诱导的凋亡细胞形态、出现时间和密度比例进行观察和比较。结果 在光镜下能观察到ＣＰＰ诱导的肾癌细胞凋亡的典型形态,用ＡＯ／ＥＢ荧光染色能区分出４种不同细胞,ＤＮＡ电泳证实有ＤＮＡ裂解的现象
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| 关 键 词： | 肾肿瘤 细胞凋亡 Cryptophycin |
Apoptosis Induced by Cryptophycin in Renal Cancer Cell Line |
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| ZHOU He-tong,GUO Zhen-hua,SU Chang-qing,et al.. Apoptosis Induced by Cryptophycin in Renal Cancer Cell Line[J]. The Practical Journal of Cancer, 2000, 15(3): 235-237 |
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| Authors: | ZHOU He-tong GUO Zhen-hua SU Chang-qing et al. |
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| Affiliation: | ZHOU He-tong,GUO Zhen-hua,SU Chang-qing,et al. Nanjing Jiangbei Hospital,Nanjing,210048 |
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| Abstract: | Objective To study a new way of inducing renal cancer cell apoptosis.Methods The renal cancer cell apoptosis induced by cryptophycin(CPP) in different concentration and different culture time was observed by HE staining,fluorescent staining and DNA electrophoresis.Results The typical characteristic of renal cancer cell apoptosis could be observed under light microscope.Four special cells could be distinguished by AO/EB fluorescent staining.The phenomenon of DNA degradation was proved by DNA electrophoresis.CPP could induce high proportion of apoptosis at 10 pmol/L drug concentration and in a culture time of 12 h.Conclusion CPP can induce renal cancer cell apoptosis sensitively. |
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| Keywords: | Renal neoplasm Apoptosis |
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