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Identification of Vibrio parahaemolyticus pandemic group-specific DNA sequence by genomic subtraction
Authors:Okura Masatoshi  Osawa Ro  Arakawa Eiji  Terajima Jun  Watanabe Haruo
Affiliation:Department of Bioscience, Graduate School of Science and Technology, Kobe University, Rokko-dai 1-1, Nada-ku, Kobe City, Japan 657-8501.
Abstract:A genomic subtraction between a pandemic Vibrio parahaemolyticus and a nonpandemic strain that seemed to be clonally related was performed. A subtractive DNA fragment was identified to be a part of a 16-kbp insertion sequence which was present in almost all pandemic strains but not in nonpandemic strains tested.
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