海南黄皮枝叶的化学成分研究

目的研究黄皮属植物海南黄皮Clausena hainanensis枝叶中的化学成分。方法综合运用硅胶柱色谱、反相硅胶柱色谱和Sephadex LH-20凝胶柱色谱以及制备型高效液相色谱等方法进行系统分离,根据化合物的理化性质及其波谱学数据鉴定化合物的结构。结果从海南黄皮枝叶的90%乙醇提取物中分离得到了18个化合物,分别鉴定为mukonine(1)、methyl carbazole-3-carboxylate(2)、lansine(3)、murrayanine(4)、3-甲酰基咔唑(5)、3-甲酰基-6-甲氧基咔唑(6)、lansamide4(7)、4-methoxy-N-methyl-2-quinolone(8)、(E)-N-(4-methoxyphenethyl)-2-methylbut-2-enamide(9)、(E)-N-甲基肉桂酰胺(10)、N-(2-羟基-2-苯乙基)肉桂酰胺(11)、N-苯甲酰酪胺(12)、aurantiamide(13)、N-methyl-2-pyrolidinone(14)、香豆酸(15)、6,8-dimethoxy-4,5-dimethyl-3-methyleneisochromanl-one(16)、8-甲氧基补骨脂素(17)和异黑麦草内酯(18)。其中化合物1~14为生物碱类化合物,15为酚酸类化合物,16为异香豆素类化合物,17为香豆素类化合物,18为倍半萜类化合物。结论首次对海南黄皮中的化学成分进行了系统研究,所有化合物均为首次从海南黄皮中分离得到,化合物12~16为首次从黄皮属植物中分离得到。

Chemical constituents of stems and leaves of Clausena hainanensis

Objective To study the chemical constituents of the stems and leaves of Clausena hainanensis. Methods The chemical constituents of C. hainanensis were separated and purified by silica gel, ODS, Sephadex LH-20 gel column chromatographies, and preparative HPLC. Their structures were identified by physicochemical properties, spectroscopic analysis, as well as comparisons with the data reported in literature. Results Eighteen compounds were isolated from the 90% ethanol extract of the stems and leaves of C. hainanensis, which were identified as mukonine (1), methyl carbazole-3-carboxylate (2), lansine (3), murrayanine (4), 3-formylcarbazole (5), 3-formyl-6-methoxycarbazole (6), lansamide 4 (7), 4-methoxy-N-methyl-2-quinolone (8), (E)-N-(4-methoxyphenethyl)-2-methylbut-2-enamide (9), (E)-N-methyl cinnamon amide (10), N-(2-hydroxy-2-phenylethyl) cinnamamide (11), N-benzoyltyramine (12), aurantiamide (13), N-methyl-2-pyrolidinone (14), coumaric acid (15), 6,8-dimethoxy-4,5-dimethyl-3-methyleneisochromanl-one (16), 8-methoxypsoralen (17), and isololiolide (18). Conclusion This is the first time reporting the chemical constituents from C. hainanensis, all compounds are isolated from C. hainanensis for the first time, and compounds 12-16 are isolated from the genus Clausena for the first time.