Proteomic analysis of hippocampus in the rat

Objective To analyze the protein expression in the rat hippocampus by the proteomic approach. Methods Proteins from hippocampal tissue homogenates of the rat were separated by two-dimensional gel electrophoresis (2-DE), and stained with colloidal Coomassie blue to produce a high-resolution map of the rat hippocampus proteome. Selected proteins from this map were digested with trypsin, and the resulting tryptic peptides were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) The mass spectrometric data were used to identify the proteins through searches of the NCBI protein sequence database. Results 37 prominent proteins with various functional characteristics were identified. The identified brain protein classes covered metabolism enzymes, cytoskeleton proteins, heat shock proteins, antioxidant proteins,signalling proteins, proteasome-related proteins, neuron-specific proteins and glial-associated proteins. Furthermore,3 hypothetical proteins, unknown proteins so far only proposed from their nucleic acid structure, were identified.Conclusion This study provides the first unbiased characterization of proteins of the rat hippocampus and will be used for future studies of differential protein expression in rat models of neurological disorders.

Proteomic analysis of hippocampus in the rat

Objective To analyze the protein expression in the rat hippocampus by the proteomic approach. Methods Proteins from hippocampal tissue homogenates of the rat were separated by two-dimensional gel. electrophoresis (2-DE), and stained with colloidal Coomassie blue to produce a high-resolution map of the rat hippocampus proteome. Selected proteins from this map were digested with trypsin, and the resulting tryptic peptides were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) . The mass spectrometric data were used to identify the proteins through searches of the NCBI prolein sequence database. Results 37 prominent proteins with various functional characteristics were identified. The identified brain protein classes covered metabolism enzymes, cytoskeleton proteins, heat shock proteins, antioxidant proteins, signalling proteins, proteasome-related proteins, neuron-specific proteins and glial-associated proteins. Furthermore, 3 hypothetical proteins, unknown proteins so far only proposed from their nucleic acid structure, were identified. Conclusion This study provides the first unbiased characterization of proteins of the rat hippocampus and will be used for future studies of differential protein expression in rat models of neurological disorders.