脐血移植后SCID鼠的免疫功能重建及抗人肝癌作用的初步研究

目的 证实脐血造血干/祖细胞移植重建SCID鼠的免疫功能对人肝癌有抑制及杀伤作用.方法 首先进行人肝癌高转移细胞株HCCLM6细胞培养,然后将HCCLM6细胞株分别在脐血移植免疫功能重建组(A组)及未移植脐血造血干/祖细胞组(B组)建立SCID鼠皮下荷瘤模型.接种肿瘤后5周处死,分别进行以下检测:(1)比较A、B两组间...

Study on the ganulocyte-macrrophage colonyatimulating dry/progenitor cells transplantation reconstruction SCID mice immune to human primary liver cancer effects

Objective to prove ganulocyte-macrrophage colonyatimulating dry/progenitor cells transplantation reconstruction SCID mice immune function to human liver has inhibitory and kill role.Methods Human liver high transfer lines HCCLM6 cell was cultured at first,and then HCCLM6 lines respectively in umbilical cord blood transplantation immunity function reconstruction group(group A) and not transplant ganulocyte-macrrophage colonyatimulating dry/progenitor cell group(group B) were established SCID mice subcutaneous tumor-burdened model.Vaccination tumor hind 5 weeks executed,and separately below detection.Compared with A and B between groups tumor growth situation differences.Conventional pathology examination: compare A and B two groups of transplantation tumor condition,and observe whether tumor metastasis.Immunohistochemical check: observation of the tumor RenYuan CD3+,CD8+ cells for tumor tissue damage.Cytotoxic test: A,B two groups of animals spleen cells and HCCLM6 cells in vitro mixed cultivating,using the MTT method observation of tumor cells kill role.Results HCCLM6 lines inoculation in group A,B group SCID mice subcutaneous tumor,all can into into tumor rate for 100 % respectively,into tumor incubation period(9.83±1.47) d,(5.16±1.17) d,two groups of comparisons P< 0.01,After inoculation 5 weeks transplant tumors secreting TSH respectively(plus or minus 94.08) 539.22 mm3,(1126.17±154.75) mm3,two groups of comparisons(P< 0.01),Were statistically significant.Pathology checks visible the group A tumor necrosis obvious,did not see the distant metastasis,Group B necrosis significantly lighter,visible pulmonary metastasis.Immunohistochemical check the group A tumor tissue which numerous RenYuan CD3+,CD8+ cells infiltration,necrotic tissue around more evident,and Group B transplant tumour inside also have tumor necrosis,but did not see RenYuan lymphocytes.Cytotoxic experiments indicated that A group of spleen cells to HCCLM6 cell damage,an obvious effect in effect target ratio for 120:1,specificity damage rate for(7.41±1.07) %,group B splenic cell of liver cancer cell damage rate for(3.97±0.27) %,two groups of comparisons(P< 0.01).Conclusion HCCLM6 is a strong vitality,metabolic activity of tumor cells subsets.RenYuan umbilical cord blood stem cells in SCID mice immune to rebuild after can give full play to HCCLM6 cell damage effect,of liver cancer growth inhibition.Umbilical cord blood stem cell transplantation may be liver cancer treatment of a new method,the application of this method is worthy of further research prospect.